FastQCFastQC Report
Thu 26 May 2016
SRR1512570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093529
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC66710.31864855944197573No Hit
GTCCTACAGTGGACATTTCTAAATT58050.27728299918463034No Hit
CTGTAGGACGTGGAATATGGCAAGA56270.2687806092010189No Hit
GTCCTAAAGTGTGTATTTCTCATTT51800.24742910177026445No Hit
GTATCAACGCAGAGTACTTTTTTTT49390.235917438927285No Hit
CCCATGTACTCTGCGTTGATACCAC49060.23434115314380646No Hit
CTTTAGGACGTGAAATATGGCGAGG48640.23233497123756108No Hit
TATCAACGCAGAGTACTTTTTTTTT30730.1467856428069542No Hit
GGTATCAACGCAGAGTACTTTTTTT30210.14430179854207895No Hit
GTCCTACAGTGTGCATTTCTCATTT29540.14110146073925892No Hit
GAGTACATGGGAAGCAGTGGTATCA28210.13474855136948188No Hit
CTGAAGGACCTGGAATATGGCGAGA26100.12466987560239193No Hit
CTGTAGGACCTGGAATATGGCGAGA22530.10761732939930614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1550.014.70525718
TAGATCG400.00526952714.254235
TAAGACG701.08822955E-412.2179124
GTCCTAT2500.012.175251
TGCGCAA550.003069923712.09045310
GGCGAGG11200.012.04044919
TAGGACC7600.011.8785254
TGTTCGC658.028E-411.69186710
AAATGTC7600.011.1256437
ATACCGT600.005874282711.0839746
AATGTCC7700.010.9801058
ATTTAGA8150.010.9707971
CCAACGA1303.284549E-810.95772219
GACCGCT700.001491056910.8577717
AGATCGT803.7698372E-410.6881186
GAAATGT8450.010.6810916
TGTCCAC8900.010.56701810
GTCCTAA15150.010.485081
TAGAAAT8500.010.39720254
GTCCTAC28550.010.3948061
Produced by FastQC (version 0.11.5)