FastQCFastQC Report
Thu 26 May 2016
SRR1512569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855343
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35290.19020741717299713No Hit
GTCCTACAGTGGACATTTCTAAATT31080.16751619511863844No Hit
GTCCTAAAGTGTGTATTTCTCATTT28270.15237074761917338No Hit
CTGTAGGACGTGGAATATGGCAAGA27530.14838226678301533No Hit
GTACATGGGAAGCAGTGGTATCAAC26040.1403514067210214No Hit
CTTTAGGACGTGAAATATGGCGAGG24450.13178156276224934No Hit
GGTATCAACGCAGAGTACTTTTTTT21780.11739069271827365No Hit
TATCAACGCAGAGTACTTTTTTTTT21040.11340221188211559No Hit
GTCCTACAGTGTGCATTTCTCATTT19150.1032154162330092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAC550.00313211712.0579893
TAGGACC5900.011.8828134
GTCTTAC1102.421457E-711.8678771
GGACTAC855.4722586E-511.1463436
GTCCTAA8700.011.0808831
GGCGAGG8550.010.95935919
TGGCGAG17900.010.73161418
GTGTAGA3000.010.7115191
TGTAGGA17550.010.5810872
TCCAACG1701.4915713E-1010.57612918
CTAGGAC2600.010.5673043
GTCTTAG2153.6379788E-1210.2755861
GGACGTG18250.010.2271516
GTCCTAC17700.010.2122541
AGGACGT18850.010.2029155
GTAGGAC17550.010.0949463
CTGTAGG17650.010.07049751
GACGTGG9800.010.0547297
GTACTAA1706.712071E-910.0420491
GTGTAAG2700.010.0420491
Produced by FastQC (version 0.11.5)