FastQCFastQC Report
Thu 26 May 2016
SRR1512569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855343
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44610.24044071635271752No Hit
GTCCTACAGTGGACATTTCTAAATT31990.1724209485793193No Hit
CTGTAGGACGTGGAATATGGCAAGA31500.16977992748510654No Hit
GTCCTAAAGTGTGTATTTCTCATTT31080.16751619511863844No Hit
GTACATGGGAAGCAGTGGTATCAAC29670.15991652217406702No Hit
GGTATCAACGCAGAGTACTTTTTTT29670.15991652217406702No Hit
TATCAACGCAGAGTACTTTTTTTTT28280.1524246460088512No Hit
CTTTAGGACGTGAAATATGGCGAGG27730.14946023457657157No Hit
GTCCTACAGTGTGCATTTCTCATTT21990.1185225589015077No Hit
CCCATGTACTCTGCGTTGATACCAC21790.11744459110795147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG7900.012.86254419
CTAGATC550.003062704812.0941353
TGGCGAG17150.012.0168118
TAGGACC7200.012.0101484
AGGACCT12000.011.0071035
TCCAACG951.3649984E-510.99670718
CGAAATC1408.370989E-910.85564913
GTCCTAC17350.010.7464441
GGCGTGC1158.8276283E-710.7382328
CGGTTTC1158.8337583E-710.73765313
GACGTGA9200.010.6355537
GGACCTG12700.010.6220176
ATGGCGA19650.010.58461817
GGACGTG18600.010.572556
TATGGCG19650.010.5365716
TAGGACT3000.010.4527884
GGCGAGA10450.010.26909819
GTAGGAC19950.010.2408273
GACGTGG9750.010.2304647
AGGACGT19000.010.2027225
Produced by FastQC (version 0.11.5)