FastQCFastQC Report
Thu 26 May 2016
SRR1512568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650361
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50800.30781144246622405No Hit
GTCCTAAAGTGTGTATTTCTCATTT49690.3010856412627298No Hit
CTGTAGGACGTGGAATATGGCAAGA46160.27969638157954535No Hit
CTTTAGGACGTGAAATATGGCGAGG40440.24503729790027756No Hit
GTATCAACGCAGAGTACTTTTTTTT37000.22419337344980883No Hit
GTCCTACAGTGTGCATTTCTCATTT26820.16250989934929388No Hit
GGTATCAACGCAGAGTACTTTTTTT23800.14421087265149868No Hit
CTGAAGGACCTGGAATATGGCGAGA22440.13597025135712731No Hit
TATCAACGCAGAGTACTTTTTTTTT22050.13360713201535906No Hit
GTACATGGGAAGCAGTGGTATCAAC19660.11912545194657412No Hit
GTCCTTCAGTGTGCATTTCTCATTT19420.11767122465933212No Hit
ATTTAGAAATGTCCACTGTAGGACG19220.11645936858663043No Hit
CTGTAGGACCTGGAATATGGCGAGA18050.10937001056132568No Hit
TTTCTAAATTTTCCACCTTTTTCAG16770.10161413169603498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC350.00225003416.197948
ACTCCGT400.00536910814.2137976
TAGGACC7250.012.5473524
AAATGTC6550.011.5735767
CATTTCG752.117521E-411.37450414
ATAATAC1701.2732926E-1111.1480763
GTCCTAA14250.010.9302891
GTCCTAT2300.010.9226241
GGCGAGG11650.010.88302719
AATGTCC7050.010.5880158
GAAATGT7350.010.5717136
TAGGACA7300.010.5143164
GTCCTAC24250.010.4010411
AGGACCT15500.010.331755
TGTAGGA27250.010.3278242
TAGAAAT7900.010.3154984
GGACCTG14950.010.2681616
TCCAACG1201.6068661E-610.24896618
GTCCTAG3150.010.2083191
CCTTATA1401.0052281E-710.1527122
Produced by FastQC (version 0.11.5)