FastQCFastQC Report
Thu 26 May 2016
SRR1512568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650361
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT53980.3270799540221806No Hit
CTGTAGGACGTGGAATATGGCAAGA52800.3199300031932408No Hit
GTCCTAAAGTGTGTATTTCTCATTT52000.3150825789024341No Hit
CTTTAGGACGTGAAATATGGCGAGG46800.28357432101219066No Hit
GTATCAACGCAGAGTACTTTTTTTT45510.2757578493432649No Hit
GGTATCAACGCAGAGTACTTTTTTT31110.18850421210874468No Hit
TATCAACGCAGAGTACTTTTTTTTT30700.1860199071597063No Hit
GTCCTACAGTGTGCATTTCTCATTT28930.1752949809162965No Hit
CTGAAGGACCTGGAATATGGCGAGA26220.15887433113118887No Hit
GTACATGGGAAGCAGTGGTATCAAC22510.13639440098257288No Hit
CTGTAGGACCTGGAATATGGCGAGA21530.13045630622633472No Hit
ATTTAGAAATGTCCACTGTAGGACG21300.12906267174272779No Hit
GTCCTTCAGTGTGCATTTCTCATTT19720.11948900876838461No Hit
TTTCTAAATTTTCCACCTTTTTCAG19420.11767122465933212No Hit
GAATATGGCAAGAAAACTGAAAATC18260.11064245943766243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8650.013.0747254
CCAACGA1103.8422513E-812.08450819
CACCTTT7600.011.86907314
TCCAACG1603.6379788E-1211.86871218
GCACTAG657.9193345E-411.7105991
GGCGAGG11800.011.58708319
GATTAGG752.0440656E-411.4178341
AGGACCT17850.011.2346385
CCACCTT8200.011.12010713
GGACCTG17350.011.06203656
TCCACCT8700.010.9183912
TAAGACT1408.296411E-910.8615974
TAGAAAT7800.010.844194
ACCTTTT8350.010.80266115
ATTTAGA8000.010.7042191
CTTTTTC9700.010.66960617
TCTACAC2700.010.5598853
TGTCCAC8150.010.49064610
AATGTCC7700.010.487188
TGTAGGA29650.010.3533172
Produced by FastQC (version 0.11.5)