FastQCFastQC Report
Thu 26 May 2016
SRR1512567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960324
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80470.41049336742293624No Hit
CTGTAGGACGTGGAATATGGCAAGA71930.3669291402849733No Hit
GTCCTAAAGTGTGTATTTCTCATTT70900.36167490680112063No Hit
CTTTAGGACGTGAAATATGGCGAGG63090.32183455387986887No Hit
GTATCAACGCAGAGTACTTTTTTTT46340.23638949479779875No Hit
GTCCTACAGTGTGCATTTCTCATTT42050.2145053572776745No Hit
CTGAAGGACCTGGAATATGGCGAGA34450.1757362558434218No Hit
CTGTAGGACCTGGAATATGGCGAGA30590.15604563327286713No Hit
GGTATCAACGCAGAGTACTTTTTTT28980.14783270520587413No Hit
GTCCTTCAGTGTGCATTTCTCATTT28070.1431906154288781No Hit
TATCAACGCAGAGTACTTTTTTTTT27920.1424254357953073No Hit
ATTTAGAAATGTCCACTGTAGGACG27360.13956876516330974No Hit
TTTCTAAATTTTCCACCTTTTTCAG25600.13059065746274595No Hit
GAATATGGCAAGAAAACTGAAAATC22330.11390974145090302No Hit
GGAATATGGCGAGAAAACTGAAAAT21140.1078393163579082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGG456.903178E-414.737966
CGGCGAG400.00542099714.19339318
CCGTCCA552.0468976E-413.748149
TAGGACC13800.013.1127864
GCGCCAC751.5174284E-512.63513113
CCAACGA1800.012.61893719
TCCAACG2500.012.49018618
ACCTTTT9300.012.13094315
CCACCTT8950.012.07043213
CACCTTT9300.011.92706214
AATGTCC9250.011.849798
AGGACGG658.210473E-411.6605035
GTCCTAC37500.011.4121761
AAACGGG752.1483403E-411.35704219
AGGACCT27500.011.2311865
CAAGTCG2450.011.2145996
TAGGACT4150.011.1863574
AGCACCG600.005996901511.0531865
GTTCTAG2000.011.0505231
CGTGCGC600.006017545711.04808210
Produced by FastQC (version 0.11.5)