FastQCFastQC Report
Thu 26 May 2016
SRR1512567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960324
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT87340.44553859464047785No Hit
CTGTAGGACGTGGAATATGGCAAGA85530.4363054270620571No Hit
GTCCTAAAGTGTGTATTTCTCATTT74630.3807023736892473No Hit
CTTTAGGACGTGAAATATGGCGAGG73010.3724384336466829No Hit
GTATCAACGCAGAGTACTTTTTTTT59220.3020929193337428No Hit
GTCCTACAGTGTGCATTTCTCATTT44610.22756442302394908No Hit
CTGAAGGACCTGGAATATGGCGAGA40340.20578230945496764No Hit
GGTATCAACGCAGAGTACTTTTTTT38900.19843658497268818No Hit
TATCAACGCAGAGTACTTTTTTTTT37790.19277425568426443No Hit
CTGTAGGACCTGGAATATGGCGAGA36640.18690787849355514No Hit
ATTTAGAAATGTCCACTGTAGGACG29700.15150556744701385No Hit
TTTCTAAATTTTCCACCTTTTTCAG29690.1514545554714425No Hit
GTCCTTCAGTGTGCATTTCTCATTT28370.14472097469601963No Hit
GAATATGGCAAGAAAACTGAAAATC27570.14064001665030884No Hit
GGAATATGGCGAGAAAACTGAAAAT23860.12171457371332493No Hit
ATTCCAGGTCCTTCAGTGTGCATTT21830.11135914267233375No Hit
ACAGTGGACATTTCTAAATTTTCCA20220.10314621460534076No Hit
TTCCAGGTCCTTCAGTGTGCATTTC19880.10141180743591366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1700.015.08345519
TAGGACC12200.014.6421764
TCCAACG2800.014.58466218
GGCGAGG19400.012.38524919
ATATCGC550.003075053712.08769515
CCACCTT9450.011.96069813
AAATGTC9250.011.8109857
CGTTTCC1950.011.68919914
CGGCGAG658.0464233E-411.68860318
GAAATGT10050.011.5333926
GCCGGTT1900.011.49899711
AATGTCC9600.011.4778738
AGGACCT25850.011.4692475
CACCTTT10000.011.3969714
TGACGAG1001.9376075E-611.39638818
ACCTTTT9950.011.358788515
ATCCCGT1950.011.2041510
GGACCTG25550.011.1184316
TACGGCG600.00589046211.07982316
TAGAAAT10550.011.0779724
Produced by FastQC (version 0.11.5)