FastQCFastQC Report
Thu 26 May 2016
SRR1512566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070971
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44740.21603392804631255No Hit
GTCCTACAGTGGACATTTCTAAATT43630.21067412339429184No Hit
CTGTAGGACGTGGAATATGGCAAGA37370.18044675661803086No Hit
GTCCTAAAGTGTGTATTTCTCATTT35250.17021001259795526No Hit
CTTTAGGACGTGAAATATGGCGAGG31260.15094368776771863No Hit
GTCCTACAGTGTGCATTTCTCATTT27890.13467112769807013No Hit
GGTATCAACGCAGAGTACTTTTTTT27850.1344779815844838No Hit
TATCAACGCAGAGTACTTTTTTTTT26240.12670385051263391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7150.011.92797854
GGCGAGG9750.011.64622519
ATTGCGA752.1187172E-411.37410217
AAGGCGT1451.2696546E-911.1099296
AAAGCCG700.001523411410.8298465
AGGACGT22950.010.694185
TTGCGAG803.918964E-410.64331218
TGGCGAG21350.010.63500418
GGACGTG22500.010.5711356
TGTAGGA24650.010.4951012
CTAAGAC2350.010.4842143
TTAGGAC13750.010.4064983
GTGTAGG3200.010.3610241
GTCCTAC22000.010.3210651
TAGGCTG2400.010.2657935
GCTAGAC750.002704067610.1078573
TAGGACG24700.010.0899534
GTAGGAC24800.010.0874793
AGGACCT14300.010.07251555
GGACCTG13550.010.07055956
Produced by FastQC (version 0.11.5)