FastQCFastQC Report
Thu 26 May 2016
SRR1512566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070971
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57480.2775509652235594No Hit
GTCCTACAGTGGACATTTCTAAATT45280.21864140057972803No Hit
CTGTAGGACGTGGAATATGGCAAGA41530.2005339524310094No Hit
GTCCTAAAGTGTGTATTTCTCATTT39150.18904175867262266No Hit
CTTTAGGACGTGAAATATGGCGAGG37150.17938445299330605No Hit
GGTATCAACGCAGAGTACTTTTTTT36540.17643897476111448No Hit
TATCAACGCAGAGTACTTTTTTTTT35780.17276919860297416No Hit
GTCCTACAGTGTGCATTTCTCATTT30590.14770849036514755No Hit
CTGTAGGACCTGGAATATGGCGAGA22180.10709951998362122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG604.0963833E-412.6654439
TAGGACC8650.012.3032524
CGTGCGC752.076777E-411.39862310
GGTATCA12600.011.32675651
GGCGAGG10000.011.30172419
TGTAGGA26100.011.0672722
AATCCCG2000.010.92183419
GCATCGT700.001491464410.8574056
AGGACGT23550.010.8133925
GTGCGCC1158.833722E-710.73783411
GGACGTG23250.010.7056346
CTGTAGG26050.010.7015031
TAGAAAT7700.010.6126974
GTAGGAC26200.010.5898323
GACGTGG11500.010.5739137
AAATGTC6500.010.5230777
TGGCGAG23150.010.37928118
GACGTGA12400.010.1895167
ACGAAAT1502.4509063E-810.131865512
GAAATGT7150.010.0981466
Produced by FastQC (version 0.11.5)