FastQCFastQC Report
Thu 26 May 2016
SRR1512565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213576
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78890.3563916486264759No Hit
CTGTAGGACGTGGAATATGGCAAGA68220.308189102158679No Hit
GTCCTAAAGTGTGTATTTCTCATTT63740.28795035724998824No Hit
CTTTAGGACGTGAAATATGGCGAGG56920.25714048218809743No Hit
GTCCTACAGTGTGCATTTCTCATTT41860.18910577274057905No Hit
GTATCAACGCAGAGTACTTTTTTTT39180.176998666411273No Hit
CTGAAGGACCTGGAATATGGCGAGA29770.13448826694904534No Hit
CTGTAGGACCTGGAATATGGCGAGA29260.13218430268488635No Hit
GTCCTTCAGTGTGCATTTCTCATTT24470.11054510890974605No Hit
GGTATCAACGCAGAGTACTTTTTTT23530.10629858654051183No Hit
TATCAACGCAGAGTACTTTTTTTTT22570.10196171263150668No Hit
ATTTAGAAATGTCCACTGTAGGACG22520.10173583378207932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12250.012.838934
GTCCTAT2700.012.6416361
TCCAACG2500.012.49074818
TGGACCG701.1190084E-412.1815155
CGTTTCC1302.7011993E-911.66862514
CCAACGA1552.7284841E-1111.60416419
GTATTAG1757.2759576E-1211.4730811
TAGGCCG752.126969E-411.3694145
AGGACGT40350.011.3412375
GGCGAGG16950.011.28167519
GGACGTG39900.011.2554356
TTAGGAC23650.011.177123
GTGTACG905.4329837E-511.1543841
GCCGGTT1701.2732926E-1111.14142211
GTGTAGG4800.011.0846691
TACGGCG600.005966329511.060883516
CCAACGT1201.3397766E-711.04431319
GGCGATA600.006033266411.04431319
TGTAGGA40350.010.9890242
GGACCGC700.001525636110.8280136
Produced by FastQC (version 0.11.5)