FastQCFastQC Report
Thu 26 May 2016
SRR1512565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213576
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT84780.38300017708901796No Hit
CTGTAGGACGTGGAATATGGCAAGA79290.3581986794218947No Hit
GTCCTAAAGTGTGTATTTCTCATTT71200.32165148158454915No Hit
CTTTAGGACGTGAAATATGGCGAGG63100.28505910797731815No Hit
GTATCAACGCAGAGTACTTTTTTTT48900.22090951473995019No Hit
GTCCTACAGTGTGCATTTCTCATTT43050.19448168935695No Hit
CTGAAGGACCTGGAATATGGCGAGA35580.16073538925250364No Hit
CTGTAGGACCTGGAATATGGCGAGA33880.15305550837197368No Hit
GGTATCAACGCAGAGTACTTTTTTT33100.149531798320907No Hit
TATCAACGCAGAGTACTTTTTTTTT32310.14596291249995483No Hit
GTCCTTCAGTGTGCATTTCTCATTT26080.11781840786130678No Hit
TTTCTAAATTTTCCACCTTTTTCAG25770.1164179589948572No Hit
ATTTAGAAATGTCCACTGTAGGACG25690.11605655283577342No Hit
GAATATGGCAAGAAAACTGAAAATC23240.10498848921383318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14050.014.4743644
TGTAGGA44100.012.4121192
GGCGAGG18700.012.33993119
AATCCCG2700.012.30983719
AGGCGTG1405.2750693E-1112.214597
GTAGGAC43750.012.0987143
GCGTGCG550.003070656412.0901189
CTGTAGG43450.012.0641121
TCCAACG2300.011.9739618
AGGACGT40950.011.7661875
GACGTGG21850.011.7394247
GGACGTG40250.011.73152356
ACGAAAT2450.011.6308412
TAGGACA11000.011.5764174
AAGGCGT1657.2759576E-1211.5162216
GGCGTGC1251.8348146E-811.3992558
GTATTAG1850.011.3131751
GCCGGTT1850.011.29604811
TTAGGAC23150.011.2886953
AGGACCT26650.011.2329815
Produced by FastQC (version 0.11.5)