FastQCFastQC Report
Thu 26 May 2016
SRR1512564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830992
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC67510.3687072362959532No Hit
CCCATGTACTCTGCGTTGATACCAC51340.280394452843049No Hit
GTCCTACAGTGGACATTTCTAAATT39530.2158938979525853No Hit
GTCCTAAAGTGTGTATTTCTCATTT35580.1943208927182642No Hit
CTGTAGGACGTGGAATATGGCAAGA34900.19060705890577348No Hit
GTATCAACGCAGAGTACTTTTTTTT34780.1899516764682751No Hit
CTTTAGGACGTGAAATATGGCGAGG30790.1681602104214546No Hit
GAGTACATGGGAAGCAGTGGTATCA30280.16537483506208656No Hit
GTCCTACAGTGTGCATTTCTCATTT21870.11944344923407639No Hit
GGTATCAACGCAGAGTACTTTTTTT21410.11693114989033268No Hit
TATCAACGCAGAGTACTTTTTTTTT20310.1109234775465977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA456.9223816E-414.73247410
TAGGACC7050.013.7066834
GTCGAGG1052.8575232E-711.71748519
TGTCGAG1407.2759576E-1011.48962618
AGGACCT13600.011.3545525
CACCTTT6150.011.25359214
GGACCTG13300.011.0408216
ATAGATC1553.2923708E-1011.001763
CCACCTT6450.010.72515913
ACCTTTT7150.010.6078515
TTCCACC6700.010.60318311
ATGTCGA1701.4370016E-1010.59644117
TTAGGAC13650.010.4801043
TCGAACT1002.5012647E-510.41053519
GTCCTAC22200.010.4044911
CTCGAAC1106.341639E-610.32223218
GACGTGA11700.010.2843057
GTCTTAG2250.010.2657661
TAGAAAT6200.010.2377484
TGTAGGA23500.010.1996652
Produced by FastQC (version 0.11.5)