FastQCFastQC Report
Thu 26 May 2016
SRR1512563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT42140.21727282898607214No Hit
GTCCTAAAGTGTGTATTTCTCATTT39550.20391885112480196No Hit
CTGTAGGACGTGGAATATGGCAAGA35880.18499641917466228No Hit
GTATCAACGCAGAGTACTTTTTTTT34820.17953108460595713No Hit
CTTTAGGACGTGAAATATGGCGAGG33920.17489070619856592No Hit
GTCCTACAGTGTGCATTTCTCATTT25810.13307574077196302No Hit
GGTATCAACGCAGAGTACTTTTTTT21030.10843017545270758No Hit
TATCAACGCAGAGTACTTTTTTTTT20760.10703806193049022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7600.013.2148674
AAAGGCG1155.5406417E-912.3584485
AAGGCGT1155.5406417E-912.3584486
TCCAACG1405.638867E-1112.16486618
ACCGCCT701.1464553E-412.1497668
AGTACCG1353.8926373E-1011.9312425
AGGACCT14550.011.8516525
GTACCGG802.9461575E-511.8435136
GGCGAGG9700.011.70805619
AGGCGTG1302.728484E-911.6613057
GTCGAGG658.2845247E-411.64801619
GGACCTG14450.011.5402546
GCGCCAC1105.1314055E-711.19953513
AGCGTGC855.6192148E-511.1174338
TGGCGAG24500.011.04492518
CCAACGA951.418996E-510.9583319
GTCCTAC20800.010.909721
ACTCCGT700.001525086910.8283556
CCAACGT700.001539659310.81601519
TGTAGGA24100.010.6935612
Produced by FastQC (version 0.11.5)