##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512563_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939497 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.747046270244294 31.0 26.0 34.0 10.0 34.0 2 28.3480041474671 31.0 28.0 34.0 16.0 34.0 3 29.5016939959175 31.0 29.0 34.0 25.0 34.0 4 33.32032429026701 35.0 35.0 37.0 28.0 37.0 5 33.61742709578824 37.0 35.0 37.0 28.0 37.0 6 33.8188128159002 37.0 35.0 37.0 30.0 37.0 7 33.76831415568057 37.0 35.0 37.0 29.0 37.0 8 33.785249990074746 37.0 35.0 37.0 29.0 37.0 9 35.30506981964911 39.0 37.0 39.0 29.0 39.0 10 35.22523623393076 39.0 35.0 39.0 29.0 39.0 11 35.271932877441934 39.0 37.0 39.0 29.0 39.0 12 35.20537232076152 39.0 35.0 39.0 29.0 39.0 13 35.178336960562454 39.0 37.0 39.0 29.0 39.0 14 36.317115726397105 40.0 37.0 41.0 29.0 41.0 15 36.25009938143756 40.0 37.0 41.0 28.0 41.0 16 36.10136339473585 40.0 37.0 41.0 27.0 41.0 17 36.05023879902882 40.0 36.0 41.0 27.0 41.0 18 36.017818021889184 40.0 36.0 41.0 27.0 41.0 19 36.02735296832117 40.0 37.0 41.0 27.0 41.0 20 35.82550166357566 40.0 36.0 41.0 27.0 41.0 21 35.81071432438411 40.0 36.0 41.0 27.0 41.0 22 35.720971468375566 40.0 36.0 41.0 27.0 41.0 23 35.576817597552356 40.0 36.0 41.0 27.0 41.0 24 35.46558514913919 40.0 36.0 41.0 26.0 41.0 25 35.348530057019936 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3376851536000594 1101 2 -0.13766006689851906 1101 3 -0.8526122151466566 1101 4 -1.1316125977879778 1101 5 -1.3738945793451975 1101 6 -1.6922388988226302 1101 7 -1.5183351855499794 1101 8 -1.6869982160135777 1101 9 -1.6621587056112759 1101 10 -2.004073457584397 1101 11 -2.181816506256652 1101 12 -1.9228601093727775 1101 13 -1.964740325116864 1101 14 -2.4255651641671605 1101 15 -2.439478193504108 1101 16 -2.667561723043299 1101 17 -2.8511255627797354 1101 18 -2.885740688358233 1101 19 -2.801298768104317 1101 20 -2.76920859425703 1101 21 -2.8044601191139193 1101 22 -2.9521307266778507 1101 23 -3.0336126329790716 1101 24 -3.2044246019588414 1101 25 -3.6354799241985383 1102 1 1.0466232570530067 1102 2 0.17042972760257769 1102 3 -0.5414392345846011 1102 4 -0.9601958325215776 1102 5 -0.941733202943098 1102 6 -0.7372407704613337 1102 7 -0.7833813041325897 1102 8 -0.7844827436330419 1102 9 -1.0263622857098866 1102 10 -1.1316953825522589 1102 11 -1.2411660189782765 1102 12 -1.2332974807289787 1102 13 -1.544550160603336 1102 14 -1.5970847942952275 1102 15 -1.6527371229267658 1102 16 -1.612877759022922 1102 17 -1.7146916316259322 1102 18 -1.8794405919161505 1102 19 -1.8425867164571201 1102 20 -1.6472469964696757 1102 21 -1.7723517306600698 1102 22 -1.8056017383562732 1102 23 -1.8501667002557056 1102 24 -2.0743898687601856 1102 25 -2.114603375078808 1103 1 2.083145836540755 1103 2 0.5962791009229633 1103 3 -0.22954431193804226 1103 4 -0.24804474787858055 1103 5 -0.6606266263906448 1103 6 -0.6870155750831586 1103 7 -0.8761328617039084 1103 8 -0.7457073088608226 1103 9 -1.0601084622114954 1103 10 -1.3722911316063602 1103 11 -1.321602598713035 1103 12 -1.4193245943246282 1103 13 -1.151953841799525 1103 14 -1.3209121421012853 1103 15 -1.5423190420277137 1103 16 -1.5958120872546502 1103 17 -1.585197978655799 1103 18 -1.5538464340143463 1103 19 -1.46967989307322 1103 20 -1.5898300631835838 1103 21 -1.6132669272011881 1103 22 -1.6982487666877972 1103 23 -1.7358209974036214 1103 24 -1.8506154842386309 1103 25 -1.724462975045995 1104 1 1.9208735414829121 1104 2 0.9240656031124388 1104 3 -0.17964618486654516 1104 4 -0.7584413384842463 1104 5 -0.7046267427161297 1104 6 -0.7976250325216867 1104 7 -0.9132323208785067 1104 8 -0.83144800700763 1104 9 -1.1714854916286228 1104 10 -1.0891152342477426 1104 11 -0.949644796665531 1104 12 -1.0665461776379175 1104 13 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0.054598709325951233 1107 11 0.18586434036313904 1107 12 0.16278046401409085 1107 13 -0.05203445277512486 1107 14 -0.01850458412739897 1107 15 -0.2808510883651323 1107 16 -0.22651044932301545 1107 17 -0.18806383782286673 1107 18 0.0026811439732981057 1107 19 -0.13454410527466365 1107 20 -0.11320203419143127 1107 21 -0.07079936269503406 1107 22 -0.03933395720072497 1107 23 -0.09244725434415813 1107 24 -0.12350100970405009 1107 25 -0.11246844408373846 1108 1 -1.848236197321917 1108 2 -3.480163572528351 1108 3 -3.255973355512669 1108 4 -2.3388250716816934 1108 5 -1.1749166167161036 1108 6 -0.601114836219125 1108 7 -0.5212527422598612 1108 8 -0.3792391217056874 1108 9 -0.5440933571010902 1108 10 -0.42295953006912157 1108 11 -0.4626308437372373 1108 12 -0.4731006937568054 1108 13 -0.5362130268219829 1108 14 -0.6934523056954376 1108 15 -0.5429493124694176 1108 16 -0.4304046252089577 1108 17 -0.47491445940381993 1108 18 -0.5428538939220147 1108 19 -0.5017791435190873 1108 20 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-0.44269067909770854 2316 15 -0.31579895571371 2316 16 -0.4327136809407506 2316 17 -0.31171436310581413 2316 18 -0.2806000742927779 2316 19 -0.29433437454976996 2316 20 -0.12295333256216878 2316 21 -0.2158486071474499 2316 22 -0.20067416066709143 2316 23 -0.4865495915433655 2316 24 -0.593021278530756 2316 25 -0.42465473160065415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72276.0 3 6.0 4 644.0 5 2789.0 6 3437.0 7 3139.0 8 2864.0 9 2725.0 10 2622.0 11 2638.0 12 2843.0 13 2788.0 14 2902.0 15 2947.0 16 2833.0 17 3176.0 18 3479.0 19 4451.0 20 5496.0 21 6688.0 22 8246.0 23 9625.0 24 10851.0 25 12430.0 26 14618.0 27 16554.0 28 19183.0 29 23048.0 30 29055.0 31 37040.0 32 48916.0 33 65558.0 34 91921.0 35 136709.0 36 228626.0 37 296657.0 38 759717.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00419847872307 19.910043563739794 12.077068235970074 25.008689721567066 2 14.390756302521007 20.147606509401598 42.07379705448338 23.38784013359402 3 18.546281109145152 24.70944994769402 29.895930465329528 26.8483384778313 4 11.955457729836057 16.896391233084472 38.98920846400247 32.158942573077 5 12.824201911889482 38.09319208906796 36.259901704650204 12.82270429439235 6 31.592337731721404 37.50215717012348 18.303963096115233 12.601542002039881 7 27.707819390331558 31.562832056372685 22.677495458659823 18.051853094635938 8 24.550553866568343 37.91631782286302 20.7457043731697 16.787423937398934 9 26.494004444138913 15.044790283472798 20.468417823488398 37.9927874488999 10 14.734323730784135 29.017852990569693 35.302880764759074 20.944942513887092 11 34.11343501368748 22.088040071387752 25.43999884258422 18.358526072340553 12 23.42290993386655 24.447363498344853 32.495408691086816 19.634317876701783 13 29.185492298675562 22.06948092422095 25.757340833609838 22.987685943493645 14 20.536728683180282 22.598036023127747 27.921077034248125 28.944158259443846 15 22.67423091727058 31.73958794631217 23.26922758557895 22.316953550838303 16 21.671166712444485 28.057586766237737 28.814216344235366 21.457030177082416 17 21.50546383657985 28.84133495378348 28.29119385541888 21.36200735421779 18 21.283836160119066 27.422095438901117 30.659566111645116 20.634502289334698 19 23.60723424502758 26.779074687426863 27.648973691728827 21.964717375816726 20 23.507451507089907 27.570472918872845 28.406023193945813 20.51605238009143 21 22.65687854569205 26.759766461648006 27.354721896829954 23.22863309582999 22 23.061449798459556 28.53973022796435 27.323092993011365 21.075726980564728 23 21.863950364358708 28.211545524563412 28.094492028636264 21.830012082441613 24 23.017038777908343 26.971168964432895 28.748748365634462 21.263043892024296 25 22.5749031596399 27.504939039471953 28.30346452015511 21.61669328073304 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1523.0 1 1523.0 2 1426.5 3 1330.0 4 1330.0 5 1330.0 6 1991.0 7 2652.0 8 2652.0 9 2652.0 10 3550.5 11 4449.0 12 4449.0 13 4449.0 14 7094.5 15 9740.0 16 9740.0 17 9740.0 18 17410.5 19 25081.0 20 25081.0 21 25081.0 22 39819.5 23 54558.0 24 54558.0 25 54558.0 26 77681.5 27 100805.0 28 100805.0 29 100805.0 30 129914.0 31 159023.0 32 159023.0 33 159023.0 34 177330.5 35 195638.0 36 195638.0 37 195638.0 38 208356.0 39 221074.0 40 221074.0 41 221074.0 42 230293.0 43 239512.0 44 239512.0 45 239512.0 46 244660.0 47 249808.0 48 249808.0 49 249808.0 50 237304.0 51 224800.0 52 224800.0 53 224800.0 54 198769.5 55 172739.0 56 172739.0 57 172739.0 58 148309.0 59 123879.0 60 123879.0 61 123879.0 62 101223.0 63 78567.0 64 78567.0 65 78567.0 66 60421.0 67 42275.0 68 42275.0 69 42275.0 70 30910.0 71 19545.0 72 19545.0 73 19545.0 74 13622.5 75 7700.0 76 7700.0 77 7700.0 78 5356.5 79 3013.0 80 3013.0 81 3013.0 82 2040.5 83 1068.0 84 1068.0 85 1068.0 86 728.0 87 388.0 88 388.0 89 388.0 90 285.5 91 183.0 92 183.0 93 183.0 94 137.0 95 91.0 96 91.0 97 91.0 98 73.5 99 56.0 100 56.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.955306968765613 2 0.2104153808951496 3 0.19448341502977318 4 0.18705880957794727 5 0.15921653913360012 6 0.21093097849597087 7 0.43047243692565645 8 0.2953343057504085 9 0.2482086850353468 10 0.21897430106878227 11 0.21402456410089832 12 0.2226866037946952 13 0.23897948798064653 14 0.2501163961583854 15 0.3374586297375041 16 0.5097713479319638 17 0.5151851227405868 18 0.22877065548438588 19 0.19953627151782136 20 0.18803844501950764 21 0.21789154610705766 22 0.29090016638334576 23 0.18716192909811152 24 0.3106475544947994 25 0.3575669361695326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1939497.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.58675847690422 #Duplication Level Percentage of deduplicated Percentage of total 1 85.36109583047228 62.814463422008394 2 10.140655761673031 14.924359726633213 3 2.428121422106956 5.360327539235454 4 0.8628574282306478 2.5397952468484566 5 0.3829300163474691 1.4089289313259097 6 0.210189860955528 0.9280314319457111 7 0.12770578992633108 0.6578218583587836 8 0.08776245884168991 0.5166523889698147 9 0.06229269751621375 0.41255259183004256 >10 0.2968175995601636 3.844816853488606 >50 0.02040707123523789 1.0333640819009262 >100 0.015290574846633353 2.1698425063663347 >500 0.002605801211861921 1.3520103491047015 >1k 0.0012676870760409181 2.0370330719836285 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4214 0.21727282898607214 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3955 0.20391885112480196 No Hit CTGTAGGACGTGGAATATGGCAAGA 3588 0.18499641917466228 No Hit GTATCAACGCAGAGTACTTTTTTTT 3482 0.17953108460595713 No Hit CTTTAGGACGTGAAATATGGCGAGG 3392 0.17489070619856592 No Hit GTCCTACAGTGTGCATTTCTCATTT 2581 0.13307574077196302 No Hit GGTATCAACGCAGAGTACTTTTTTT 2103 0.10843017545270758 No Hit TATCAACGCAGAGTACTTTTTTTTT 2076 0.10703806193049022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0311952016424878E-4 0.0 3 0.0 0.0 0.0 1.0311952016424878E-4 0.0 4 0.0 0.0 0.0 1.0311952016424878E-4 0.0 5 0.0 0.0 0.0 1.0311952016424878E-4 5.155976008212439E-5 6 0.0 0.0 0.0 1.0311952016424878E-4 5.155976008212439E-5 7 0.0 0.0 0.0 1.0311952016424878E-4 5.155976008212439E-5 8 0.0 0.0 0.0 1.0311952016424878E-4 5.155976008212439E-5 9 0.0 0.0 0.0 1.0311952016424878E-4 5.155976008212439E-5 10 0.0 0.0 0.0 1.0311952016424878E-4 5.155976008212439E-5 11 0.0 0.0 0.0 1.0311952016424878E-4 5.155976008212439E-5 12 0.0 0.0 0.0 1.0311952016424878E-4 1.5467928024637316E-4 13 0.0 0.0 0.0 1.5467928024637316E-4 2.5779880041062195E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 760 0.0 13.214867 4 AAAGGCG 115 5.5406417E-9 12.358448 5 AAGGCGT 115 5.5406417E-9 12.358448 6 TCCAACG 140 5.638867E-11 12.164866 18 ACCGCCT 70 1.1464553E-4 12.149766 8 AGTACCG 135 3.8926373E-10 11.931242 5 AGGACCT 1455 0.0 11.851652 5 GTACCGG 80 2.9461575E-5 11.843513 6 GGCGAGG 970 0.0 11.708056 19 AGGCGTG 130 2.728484E-9 11.661305 7 GTCGAGG 65 8.2845247E-4 11.648016 19 GGACCTG 1445 0.0 11.540254 6 GCGCCAC 110 5.1314055E-7 11.199535 13 AGCGTGC 85 5.6192148E-5 11.117433 8 TGGCGAG 2450 0.0 11.044925 18 CCAACGA 95 1.418996E-5 10.95833 19 GTCCTAC 2080 0.0 10.90972 1 ACTCCGT 70 0.0015250869 10.828355 6 CCAACGT 70 0.0015396593 10.816015 19 TGTAGGA 2410 0.0 10.693561 2 >>END_MODULE