FastQCFastQC Report
Thu 26 May 2016
SRR1512563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43760.2256255101193763No Hit
GTCCTAAAGTGTGTATTTCTCATTT42010.21660255210500454No Hit
CTGTAGGACGTGGAATATGGCAAGA41040.21160125537703847No Hit
GTATCAACGCAGAGTACTTTTTTTT40540.20902326737293228No Hit
CTTTAGGACGTGAAATATGGCGAGG38460.1982988372758504No Hit
GTCCTACAGTGTGCATTTCTCATTT28490.14689375647397238No Hit
TATCAACGCAGAGTACTTTTTTTTT26370.13596308733656198No Hit
GGTATCAACGCAGAGTACTTTTTTT25670.1323539041308133No Hit
CTGTAGGACCTGGAATATGGCGAGA22040.11363771122100215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9400.012.8395344
AATCCCG2300.012.79819719
TATGTCG951.0456042E-611.99485416
TGTAGGA26050.011.7468532
AGGACGT23350.011.51818755
GGACGTG22850.011.517716
GGCGAGG10600.011.46618819
GGACCTG16300.011.30802256
GACGTGA11700.011.1251847
GTAGGAC26350.011.1081953
AGGACCT16950.011.10145955
TTAGGAC13250.011.0453183
CTGTAGG26850.010.9843141
GTCCTAA12600.010.7974461
GTAGAAC5850.010.7216463
TGGCGAG24150.010.69492918
TAGGACG25250.010.6888344
GACGTGG12400.010.65039357
AAAGGCG1252.2291897E-710.6439455
AATGTCC6200.010.5724098
Produced by FastQC (version 0.11.5)