FastQCFastQC Report
Thu 26 May 2016
SRR1512562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025444
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55370.27337215938826254No Hit
CTGTAGGACGTGGAATATGGCAAGA50530.2494761642385571No Hit
GTCCTAAAGTGTGTATTTCTCATTT48020.23708381964645778No Hit
CTTTAGGACGTGAAATATGGCGAGG43400.21427400609446617No Hit
GTACATGGGAAGCAGTGGTATCAAC37730.1862801440079311No Hit
GTATCAACGCAGAGTACTTTTTTTT34130.16850626331806753No Hit
CCCATGTACTCTGCGTTGATACCAC28440.14041365744992207No Hit
GTCCTACAGTGTGCATTTCTCATTT25440.12560209020836913No Hit
CTGAAGGACCTGGAATATGGCGAGA22850.1128147704898284No Hit
TATCAACGCAGAGTACTTTTTTTTT20790.10264416098396204No Hit
GGTATCAACGCAGAGTACTTTTTTT20340.1004224258977291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.013.4602734
ACCGTGC905.7748184E-712.598218
GTGTAGG3750.012.3151881
GACGTGG16150.011.6755767
GTCCTAC25250.011.6101011
TGTAGGA30700.011.5421812
GGCGAGG12550.011.46252519
GTAGGAC28600.011.3627313
AGGACGT30100.011.1427255
GGACGTG29900.011.12330156
CTGTAGG27800.011.1229621
GCACCGT600.005991159511.0546396
ACGTGGA17200.010.8769448
TAAGACG700.001525603110.8279584
GTCCTAA14400.010.8064671
CCGTGCT1053.6844613E-610.8022119
AGGACCT17750.010.7822075
AAGGCGT1159.118885E-710.7113286
GGACCTG17550.010.6902016
TCCTACA28900.010.6874572
Produced by FastQC (version 0.11.5)