FastQCFastQC Report
Thu 26 May 2016
SRR1512562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025444
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58110.2869000574688809No Hit
CTGTAGGACGTGGAATATGGCAAGA56950.2811729181354804No Hit
GTCCTAAAGTGTGTATTTCTCATTT50880.25120418041673825No Hit
CTTTAGGACGTGAAATATGGCGAGG50050.24710631347990858No Hit
GTATCAACGCAGAGTACTTTTTTTT45650.2253826815256309No Hit
GTACATGGGAAGCAGTGGTATCAAC43630.21540955958298524No Hit
CCCATGTACTCTGCGTTGATACCAC33970.16771631306518472No Hit
GGTATCAACGCAGAGTACTTTTTTT29510.14569644976607599No Hit
TATCAACGCAGAGTACTTTTTTTTT29170.1440178054787No Hit
CTGAAGGACCTGGAATATGGCGAGA28160.13903124450737717No Hit
GTCCTACAGTGTGCATTTCTCATTT25960.12816942853023833No Hit
GTCCTTCAGTGTGCATTTCTCATTT20590.10165672316785851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC350.002172523216.283458
TAGGACC8400.012.5583384
ATGACGT701.08735505E-412.2189245
TGTACCG550.00306028412.0955015
TAGGACT3950.011.5486874
AATGTCC7700.011.2257118
AGGACCT18650.011.2106895
GGCGAGG12150.011.09832219
GGACCTG18200.010.9621796
CACCTTT7300.010.92782814
GGACGTA1053.464871E-610.8577786
GGCGTGC700.001493472610.8556338
TTATACT2550.010.8080244
ACCTTTT7450.010.7075415
AAATGTC7950.010.5150397
GAAATGT8050.010.503726
CCAACGA1106.076687E-610.35938119
TTAGGAC17700.010.2547983
GTCTTAT1303.7478821E-710.2493421
GTATAGG2053.6379788E-1210.213631
Produced by FastQC (version 0.11.5)