FastQCFastQC Report
Thu 26 May 2016
SRR1512561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714390
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36510.21296204480894082No Hit
GTACATGGGAAGCAGTGGTATCAAC32620.19027175846802652No Hit
GTCCTAAAGTGTGTATTTCTCATTT29050.16944802524513092No Hit
GTCCTACAGTGGACATTTCTAAATT28840.16822309976143118No Hit
CTGTAGGACGTGGAATATGGCAAGA27290.15918198309602832No Hit
CTTTAGGACGTGAAATATGGCGAGG24430.1424996646037366No Hit
CCCATGTACTCTGCGTTGATACCAC24300.1417413773995415No Hit
GGTATCAACGCAGAGTACTTTTTTT22620.1319419735299436No Hit
TATCAACGCAGAGTACTTTTTTTTT21970.1281505375089682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA604.2032346E-412.62659311
GTCCTAC14450.011.4645841
GGCGAAA752.1470494E-411.35760719
GTCCTAA8250.011.196341
GTATAAT2300.010.9132581
TAGGACC6200.010.8509774
TCTATAC1553.8180588E-910.3927913
TCCAACG1553.9035513E-910.37878318
TCCTACA17250.010.3272262
GAAATGT5800.010.2926586
CTAAGAC1752.4738256E-1010.287993
AATGTCC5800.010.264958
AGGACGT17150.010.2213865
AAGTCGT1606.4337655E-910.0680167
AGTCGTC1606.724804E-910.0409138
GTCTTAT1302.0915231E-610.0401961
GGACGTG17450.09.9916536
TGGCGAG16450.09.95197918
CTGGACT2107.2759576E-129.9267174
CTTATCC1151.0579322E-59.8877713
Produced by FastQC (version 0.11.5)