FastQCFastQC Report
Thu 26 May 2016
SRR1512561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714390
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44780.26120077695273536No Hit
GTACATGGGAAGCAGTGGTATCAAC38780.22620290598988563No Hit
GTCCTAAAGTGTGTATTTCTCATTT32490.18951347126383147No Hit
CTGTAGGACGTGGAATATGGCAAGA30530.1780808334159672No Hit
GTCCTACAGTGGACATTTCTAAATT30360.1770892270720198No Hit
GGTATCAACGCAGAGTACTTTTTTT29650.1729478123414159No Hit
TATCAACGCAGAGTACTTTTTTTTT29500.17207286556734464No Hit
CTTTAGGACGTGAAATATGGCGAGG28630.16699817427773142No Hit
CCCATGTACTCTGCGTTGATACCAC28050.16361504675132263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC1500.014.56355813
TCCAACG1403.6379788E-1212.88860218
ACGAAAT1650.012.665441512
GGCGAGG7150.012.35184419
ACCGTCA600.00587647911.0832318
TCCTATA2600.010.9639972
TAGGACC5950.010.8598954
GGACGTG14150.010.7428916
GACGAAA2050.010.65781511
AGGACGT14350.010.5956375
CCAACGT909.55301E-510.55145619
GACGTGA8050.010.5033157
ATTATAC1909.094947E-1210.5023553
TGGCGAG16050.010.29566718
CTAGTAC1201.5191908E-610.2939743
AAGGCGT1201.5220376E-610.2924726
TTATACT2350.010.1089444
GTCCTAT2750.010.03126051
GTATTAC951.6225429E-410.0130551
GGGTAAT951.6225429E-410.0130551
Produced by FastQC (version 0.11.5)