FastQCFastQC Report
Thu 26 May 2016
SRR1512560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163310
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76240.3524229074889868No Hit
GTCCTAAAGTGTGTATTTCTCATTT70070.3239017986326509No Hit
CTGTAGGACGTGGAATATGGCAAGA67320.3111897971164558No Hit
CTTTAGGACGTGAAATATGGCGAGG60440.27938668059593863No Hit
GTATCAACGCAGAGTACTTTTTTTT50440.23316122053704738No Hit
GTCCTACAGTGTGCATTTCTCATTT39930.1845782620151527No Hit
CTGAAGGACCTGGAATATGGCGAGA33280.15383833107599004No Hit
CTGTAGGACCTGGAATATGGCGAGA30880.14274422066185613No Hit
GGTATCAACGCAGAGTACTTTTTTT29890.1381679001160259No Hit
TATCAACGCAGAGTACTTTTTTTTT29880.13812167465596703No Hit
GTCCTTCAGTGTGCATTTCTCATTT27210.12577947682024304No Hit
ATTTAGAAATGTCCACTGTAGGACG25840.11944658879217496No Hit
TTTCTAAATTTTCCACCTTTTTCAG24090.111357133281869No Hit
GAATATGGCAAGAAAACTGAAAATC22090.10211204127009074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12100.014.5651394
GGCGTGC1051.4842954E-913.4995558
CGGAATA604.1960122E-412.62945211
GGACCTG23300.011.9560746
AGGACCT24550.011.8101935
GTCGAGG1451.03682396E-1011.746716519
AATGTCC10000.011.4341238
AAATGTC9950.011.42760857
TATGTCG1501.8371793E-1011.37575916
CGTGCGC752.1329054E-411.36597710
TGTAGGA40850.011.3428992
GGCGAGG17100.011.28875419
ACCTTTT9250.011.27327515
GTCTTAT2050.011.264411
GAAATGT10850.011.0910116
TTTGGCG600.00597074211.05976616
CGTTTAC600.00597074211.05976614
CTGTAGG38500.011.0570571
GACCGTG600.00599144311.0546247
GCACCGA600.00599144311.0546246