FastQCFastQC Report
Thu 26 May 2016
SRR1512560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163310
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT82140.3796959289237326No Hit
CTGTAGGACGTGGAATATGGCAAGA76690.3545030531916369No Hit
GTCCTAAAGTGTGTATTTCTCATTT75960.3511285946073378No Hit
CTTTAGGACGTGAAATATGGCGAGG68980.31886322348623175No Hit
GTATCAACGCAGAGTACTTTTTTTT62300.2879846161668924No Hit
GTCCTACAGTGTGCATTTCTCATTT42660.19719781261122998No Hit
TATCAACGCAGAGTACTTTTTTTTT40690.18809139697962843No Hit
GGTATCAACGCAGAGTACTTTTTTT40100.18536409483615385No Hit
CTGAAGGACCTGGAATATGGCGAGA38610.17847650128737905No Hit
CTGTAGGACCTGGAATATGGCGAGA35250.16294474670759163No Hit
ATTTAGAAATGTCCACTGTAGGACG29960.13849147833643816No Hit
TTTCTAAATTTTCCACCTTTTTCAG28180.1302633464459555No Hit
GTCCTTCAGTGTGCATTTCTCATTT27750.1282756516634232No Hit
GAATATGGCAAGAAAACTGAAAATC26030.1203248725332939No Hit
GTACATGGGAAGCAGTGGTATCAAC25040.11574855198746366No Hit
GGAATATGGCGAGAAAACTGAAAAT23830.11015527132033781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT1800.013.19344811
TAGGACC12150.013.1393684
TCCAACG2650.012.90021218
TGCCGGT1950.012.17856810
CCAACGT1501.4551915E-1112.02797519
CCGGTTT2000.011.8732812
GGCGAGG17650.011.78228819
CACCTTT9350.011.27487814
AGGACCT23950.011.2687145
CCAACGA1354.7639332E-911.25424619
TGTAGGA41100.011.1903852
ACCTTTT9000.011.18571815
GGACCTG24500.011.0514646
CGGTTTC2250.010.97593513
CTGTAGG41200.010.8086921
TCGAACT1605.493348E-1010.68274119
AATGTCC9750.010.6199578
AATCCCG2700.010.55085619
GTCCTAA22600.010.5258231
GTAGGAC42800.010.5016813
Produced by FastQC (version 0.11.5)