FastQCFastQC Report
Thu 26 May 2016
SRR1512559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824353
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT42060.23054748724616342No Hit
GTCCTAAAGTGTGTATTTCTCATTT39290.2153640222040362No Hit
CTGTAGGACGTGGAATATGGCAAGA37840.20741599898703814No Hit
GTATCAACGCAGAGTACTTTTTTTT35510.1946443478866206No Hit
CTTTAGGACGTGAAATATGGCGAGG35100.1923969758045729No Hit
GTCCTACAGTGTGCATTTCTCATTT21820.11960404592751513No Hit
TATCAACGCAGAGTACTTTTTTTTT21040.11532855757630238No Hit
GGTATCAACGCAGAGTACTTTTTTT20390.11176565061695844No Hit
CTGAAGGACCTGGAATATGGCGAGA19090.10463983669827055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA400.005373931514.2119882
TAGGACC6950.012.9495454
GGCGAGG10350.011.7979419
TGGCGAA658.290206E-411.64699518
TGTCGAG1157.4667696E-811.52039718
TCCAACG1751.8189894E-1211.3558218
GCCTCGA1201.3114186E-711.06108616
TATGTCG1201.3114186E-711.06108616
AGGACCT14500.011.0416615
GTGTAGG2950.010.8917491
CTACACT3400.010.8667944
GGACCTG14050.010.6536026
TGGCGAG24350.010.45419518
TGTAGGA23100.010.3359922
GTTTAGG1955.638867E-1110.2982881
GTATAGA2450.010.2457471
GTCCTAA12750.010.237711
CTCGAAC1401.0254007E-710.13912518
TAGGACA6550.010.1244744
GTCCTAC21650.010.0640191
Produced by FastQC (version 0.11.5)