FastQCFastQC Report
Thu 26 May 2016
SRR1512559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824353
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44700.24501837089642192No Hit
GTCCTAAAGTGTGTATTTCTCATTT43210.23685109186654119No Hit
CTGTAGGACGTGGAATATGGCAAGA42330.23202746398312168No Hit
GTATCAACGCAGAGTACTTTTTTTT42270.2316985802637976No Hit
CTTTAGGACGTGAAATATGGCGAGG39290.2153640222040362No Hit
TATCAACGCAGAGTACTTTTTTTTT28160.15435609226942373No Hit
GGTATCAACGCAGAGTACTTTTTTT27350.149916162058549No Hit
GTCCTACAGTGTGCATTTCTCATTT23970.13138904586996047No Hit
CTGAAGGACCTGGAATATGGCGAGA21640.11861739476954294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA250.00601885619.0074352
TAGGACC7700.013.3295244
AGAACCG550.003060448412.0953075
AAAGGCG1103.7956852E-812.0953075
GTACTAG1353.601599E-1011.9844091
GTCCTAA11850.011.4846871
AAGGCGT1104.967169E-711.2276616
GTGCGCC855.3278713E-511.175631511
TAGAACG600.005860430711.0873654
AAGGCGA600.0058952211.07855217
CACCTTT6100.011.05291114
GCGTGCG700.001492209410.85662659
GGCGAGG9950.010.78429119
CCAACGA803.786416E-410.6828919
GGTATCA11900.010.6366821
TGTAGGA24500.010.4346922
AGGACGT24150.010.2314645
AGGACCT15450.010.2108345
TGGCGAG22650.010.18765718
AATCCCG1409.749601E-810.17418219
Produced by FastQC (version 0.11.5)