FastQCFastQC Report
Thu 26 May 2016
SRR1512558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088648
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51210.2451825295597918No Hit
CTGTAGGACGTGGAATATGGCAAGA44050.21090198061138113No Hit
GTCCTAAAGTGTGTATTTCTCATTT41920.2007039960778456No Hit
CTTTAGGACGTGAAATATGGCGAGG36860.17647779807799113No Hit
GTATCAACGCAGAGTACTTTTTTTT36170.17317422562346552No Hit
GTACATGGGAAGCAGTGGTATCAAC33360.15972054649706413No Hit
GTCCTACAGTGTGCATTTCTCATTT27670.1324780432126428No Hit
CCCATGTACTCTGCGTTGATACCAC25620.12266308157238559No Hit
GGTATCAACGCAGAGTACTTTTTTT22100.10581007426813901No Hit
TATCAACGCAGAGTACTTTTTTTTT21680.10379920407842776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8200.014.0968934
ACGTTGG500.001533300413.26111311
TGTCGAG951.091943E-611.95196718
TTAACCT1701.8189894E-1211.7043634
GTCTTAC1903.6379788E-1211.0986861
GGACCTG14750.011.0490346
GGTCTAC1653.5470293E-1010.9545481
GTAGGAC26900.010.8134313
AAATGTC6400.010.8078977
AGGACCT15750.010.7683685
GTATTAG1404.2986358E-810.7589311
TTAGGAC15100.010.7299213
GTCGTAC750.001669985910.7111141
ACGAAAT1951.8189894E-1210.68712512
TAGGACA7450.010.6831774
GGCGAGG11450.010.66175519
CTAGGAC3200.010.659333
TGTAGGA29050.010.632832
GGCGTGC1451.567787E-810.4275128
AGGACGT26650.010.3104635
Produced by FastQC (version 0.11.5)