FastQCFastQC Report
Thu 26 May 2016
SRR1512558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088648
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52920.25336964390361616No Hit
CTGTAGGACGTGGAATATGGCAAGA49840.23862326251240037No Hit
GTATCAACGCAGAGTACTTTTTTTT46490.22258417885637022No Hit
GTCCTAAAGTGTGTATTTCTCATTT45330.21703034690383444No Hit
CTTTAGGACGTGAAATATGGCGAGG42040.20127853041776306No Hit
GTACATGGGAAGCAGTGGTATCAAC39140.18739395053642355No Hit
GTCCTACAGTGTGCATTTCTCATTT31050.1486607604536523No Hit
TATCAACGCAGAGTACTTTTTTTTT29650.1419578598212815No Hit
GGTATCAACGCAGAGTACTTTTTTT29600.14171847051298256No Hit
CCCATGTACTCTGCGTTGATACCAC28480.13635615000708592No Hit
CTGTAGGACCTGGAATATGGCGAGA23660.11327902068706648No Hit
CTGAAGGACCTGGAATATGGCGAGA23550.11275236420880877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACT400.0052693614.2542964
TAGGACC8650.013.1831634
GCGTGCG655.454755E-513.1537139
GGCGAGG12200.012.21972519
TATTGCG550.003079761312.08528616
TCCAACG2550.011.5436518
CCAACGA1850.011.29204519
AAAGGCG1104.948324E-711.23119455
TATGTCG1201.2841338E-711.07817916
GTCTAAG3100.011.050411
ACCGGGC700.001491899810.8570338
AGGACGT26650.010.8048995
AATGTCC6850.010.6787248
GACGTGA15150.010.6607257
TTAGGAC17150.010.58336453
GGACGTG26700.010.5688296
TACACCG909.484101E-510.5592435
AAGGCGT909.504857E-510.5569676
TGTAGGA28150.010.4647142
GGCGTGT1106.047998E-610.3635328
Produced by FastQC (version 0.11.5)