FastQCFastQC Report
Thu 26 May 2016
SRR1512557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886979
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT49950.2647088282381521No Hit
CTGTAGGACGTGGAATATGGCAAGA43520.23063319729578335No Hit
GTCCTAAAGTGTGTATTTCTCATTT41820.22162408802641684No Hit
CTTTAGGACGTGAAATATGGCGAGG35870.19009220558363393No Hit
GTATCAACGCAGAGTACTTTTTTTT33150.17567763075264747No Hit
GTCCTACAGTGTGCATTTCTCATTT28650.15182998856903018No Hit
GGTATCAACGCAGAGTACTTTTTTT20140.10673144746178945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9100.014.1602914
TGTAGGA28550.011.1846292
TCGAGAC600.00599208511.0543453
CTGTAGG26900.011.01400851
GTAGGAC28850.011.0023443
TAGTACC1303.396417E-810.9325774
AGGACCT17050.010.8919685
TAGAAAT7500.010.86455254
GGACGTG25800.010.8337046
TCGAACT1053.6278998E-610.81606619
GTATAAG1955.456968E-1210.8158431
AGGACGT26550.010.7774765
CTAGGAC2550.010.7756643
GACGTGG13150.010.7358197
GTCCTAA12200.010.6195361
GGCGAGG11950.010.61241619
CCAACGA1451.5312253E-810.44309719
GGACCTG16800.010.4336716
GTCCTAC25850.010.3735261
GTCTTAT2051.2732926E-1110.288241
Produced by FastQC (version 0.11.5)