##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512557_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886979 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.438770648745958 31.0 26.0 33.0 7.0 34.0 2 28.03683718790723 31.0 27.0 34.0 16.0 34.0 3 29.1714762061475 31.0 28.0 34.0 22.0 34.0 4 32.965635017665804 35.0 35.0 37.0 27.0 37.0 5 33.26115287981477 37.0 35.0 37.0 28.0 37.0 6 33.46072743787822 37.0 35.0 37.0 28.0 37.0 7 33.42005978868869 37.0 35.0 37.0 28.0 37.0 8 33.42942926232883 37.0 35.0 37.0 28.0 37.0 9 34.918940274375075 39.0 35.0 39.0 28.0 39.0 10 34.843422210846015 39.0 35.0 39.0 27.0 39.0 11 34.874369561081494 39.0 35.0 39.0 27.0 39.0 12 34.81576689512708 39.0 35.0 39.0 27.0 39.0 13 34.77287770558125 39.0 35.0 39.0 27.0 39.0 14 35.875670582449516 40.0 36.0 41.0 27.0 41.0 15 35.80526598335223 40.0 36.0 41.0 27.0 41.0 16 35.659489586264606 40.0 36.0 41.0 27.0 41.0 17 35.60222450806289 40.0 36.0 41.0 27.0 41.0 18 35.56697981270592 40.0 36.0 41.0 26.0 41.0 19 35.571473238440916 40.0 36.0 41.0 26.0 41.0 20 35.36979160870365 40.0 36.0 41.0 25.0 41.0 21 35.344744695092 40.0 36.0 41.0 25.0 41.0 22 35.25174313015672 40.0 36.0 41.0 24.0 41.0 23 35.10141925267849 40.0 36.0 41.0 24.0 41.0 24 34.983142366714205 40.0 36.0 41.0 22.0 41.0 25 34.8597075007194 40.0 36.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.851675457879967 1101 2 -0.3526679255830878 1101 3 -1.213932780638185 1101 4 -1.6528515222719875 1101 5 -1.7002171770058112 1101 6 -1.9020153891620026 1101 7 -1.989542428267292 1101 8 -1.912964733889872 1101 9 -1.9161078558096065 1101 10 -2.423401093309394 1101 11 -2.2582947167876597 1101 12 -2.298259172704583 1101 13 -2.463805871643139 1101 14 -3.171985505942537 1101 15 -3.015548822379607 1101 16 -3.127682542803335 1101 17 -3.4291527868713985 1101 18 -3.2889639044699948 1101 19 -3.317845558336572 1101 20 -3.1955070932886898 1101 21 -3.385050514786343 1101 22 -3.501161606842132 1101 23 -3.7583821442384 1101 24 -3.7843904671821633 1101 25 -3.938507284970303 1102 1 0.7016265808449305 1102 2 -0.16390521855967677 1102 3 -0.7184818983708254 1102 4 -1.2553502182139198 1102 5 -1.236566270333455 1102 6 -1.119002337853857 1102 7 -1.2534953132191973 1102 8 -1.2099847768265732 1102 9 -1.2648227719543144 1102 10 -1.3507404527989806 1102 11 -1.6390228169929557 1102 12 -1.6061193240423677 1102 13 -1.6279321393283794 1102 14 -1.9794856165695691 1102 15 -1.9070401954823524 1102 16 -1.9495354718719682 1102 17 -1.9515030682663408 1102 18 -2.2144771803845416 1102 19 -2.1245537134933343 1102 20 -2.300976359757705 1102 21 -2.3332797447283227 1102 22 -2.2869699686367824 1102 23 -2.4392372308694235 1102 24 -2.596162256349835 1102 25 -2.60627351730853 1103 1 2.2314329873535 1103 2 0.555545683950271 1103 3 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82106.0 3 3.0 4 741.0 5 3050.0 6 3697.0 7 3354.0 8 3073.0 9 2912.0 10 2935.0 11 2855.0 12 2863.0 13 3034.0 14 2977.0 15 3196.0 16 3244.0 17 3367.0 18 3926.0 19 4531.0 20 5928.0 21 7120.0 22 8642.0 23 10335.0 24 11823.0 25 13369.0 26 15410.0 27 17451.0 28 20307.0 29 24300.0 30 30050.0 31 38181.0 32 49910.0 33 66432.0 34 92452.0 35 135904.0 36 219601.0 37 283658.0 38 704242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02697494097295 19.8373727185128 12.071271997791063 25.06438034272319 2 14.262399731484313 20.157848560551987 42.341620356208146 23.23813135175555 3 18.683777058679222 24.81300664499459 29.64456659915825 26.85864969716794 4 11.986931977470244 16.974358810986228 38.982391195385304 32.05631801615822 5 12.800567760483212 38.16485331173267 36.27031896185186 12.764259965932249 6 31.47062135130312 37.57063707203494 18.374189532541916 12.58455204412002 7 27.774865194715197 31.41917191685637 22.74809574727981 18.057867141148627 8 24.381487385760614 38.148100701290154 20.803913574347227 16.666498338602 9 26.48265968892372 15.1271268612111 20.474982400680062 37.91523104918512 10 14.802939076933535 28.963207362776505 35.31332591163648 20.920527648653472 11 34.03194444960806 22.078720819871776 25.590188391841863 18.299146338678298 12 23.577419303436077 24.488306500873758 32.52954580462853 19.40472839106163 13 29.283676602616886 22.24762137495418 25.75300548770446 22.715696534724472 14 20.292939715072347 22.82996893997435 27.880331236099764 28.996760108853543 15 22.516589393954703 32.21758946361447 23.164903813686177 22.10091732874465 16 21.39912281169002 28.165479007245274 29.10253856208872 21.332859618975988 17 21.355842999129575 29.106538435723124 28.47577131935322 21.061847245794088 18 21.171786320005822 27.432333305181483 31.02445652318252 20.371423851630176 19 23.575610755974804 26.83088726490289 27.679780453897123 21.91372152522518 20 23.597612161558732 27.674862759819195 28.436207939249552 20.29131713937252 21 22.453325912144955 26.931584680415337 27.399640022794664 23.215449384645044 22 22.886952454603197 28.926129372589976 27.356554599740097 20.83036357306673 23 21.641533887969448 28.419180873708516 28.291963935378465 21.647321302943563 24 23.001207321760553 27.033374924176893 29.05201052831358 20.913407225748966 25 22.46986926505473 27.713521333517715 28.382673630687076 21.433935770740476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1575.0 1 1575.0 2 1404.5 3 1234.0 4 1234.0 5 1234.0 6 1864.0 7 2494.0 8 2494.0 9 2494.0 10 3392.5 11 4291.0 12 4291.0 13 4291.0 14 6977.0 15 9663.0 16 9663.0 17 9663.0 18 16901.0 19 24139.0 20 24139.0 21 24139.0 22 38257.0 23 52375.0 24 52375.0 25 52375.0 26 75828.0 27 99281.0 28 99281.0 29 99281.0 30 127941.0 31 156601.0 32 156601.0 33 156601.0 34 175672.0 35 194743.0 36 194743.0 37 194743.0 38 207197.0 39 219651.0 40 219651.0 41 219651.0 42 227777.5 43 235904.0 44 235904.0 45 235904.0 46 241289.5 47 246675.0 48 246675.0 49 246675.0 50 232365.5 51 218056.0 52 218056.0 53 218056.0 54 190691.0 55 163326.0 56 163326.0 57 163326.0 58 139271.5 59 115217.0 60 115217.0 61 115217.0 62 93687.5 63 72158.0 64 72158.0 65 72158.0 66 55664.0 67 39170.0 68 39170.0 69 39170.0 70 28627.5 71 18085.0 72 18085.0 73 18085.0 74 12702.0 75 7319.0 76 7319.0 77 7319.0 78 5069.0 79 2819.0 80 2819.0 81 2819.0 82 1999.5 83 1180.0 84 1180.0 85 1180.0 86 813.5 87 447.0 88 447.0 89 447.0 90 359.0 91 271.0 92 271.0 93 271.0 94 219.0 95 167.0 96 167.0 97 167.0 98 152.5 99 138.0 100 138.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.954968232290874 2 0.21383385824643517 3 0.1991013148530005 4 0.19094012175016256 5 0.16354183061920669 6 0.21807343907907828 7 0.43233125540877776 8 0.30376596665887645 9 0.25458682900021673 10 0.22316093607824997 11 0.21982226617254355 12 0.2283544225982377 13 0.24319295551248846 14 0.25527576088552123 15 0.3396964142155265 16 0.5096506108441059 17 0.5162219611347026 18 0.2295733020876226 19 0.20249297951911494 20 0.19088712698975452 21 0.216324611985613 22 0.2922131088899241 23 0.18982723178159375 24 0.315636792990277 25 0.362431166430575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1886979.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.87180604897276 #Duplication Level Percentage of deduplicated Percentage of total 1 86.71717359196265 64.9267140229253 2 9.295887319080562 13.919997448146102 3 2.183495687362327 4.9044679683888175 4 0.7364528374024893 2.205582160248594 5 0.33114017094154674 1.2396531326879587 6 0.18214736950482802 0.8182621513137635 7 0.11963460845516652 0.6270081440700017 8 0.07726667888592131 0.46280766364759673 9 0.05706497288006449 0.38453018234994707 >10 0.2620691993422699 3.497159027607318 >50 0.01851113513505092 0.9745866574688632 >100 0.01545433261448926 2.4197591268985708 >500 0.00242060151365103 1.3548367914232047 >1k 0.0012814949189917189 2.2646355228238884 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4995 0.2647088282381521 No Hit CTGTAGGACGTGGAATATGGCAAGA 4352 0.23063319729578335 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4182 0.22162408802641684 No Hit CTTTAGGACGTGAAATATGGCGAGG 3587 0.19009220558363393 No Hit GTATCAACGCAGAGTACTTTTTTTT 3315 0.17567763075264747 No Hit GTCCTACAGTGTGCATTTCTCATTT 2865 0.15182998856903018 No Hit GGTATCAACGCAGAGTACTTTTTTT 2014 0.10673144746178945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.299476040803846E-5 0.0 5 0.0 0.0 0.0 5.299476040803846E-5 0.0 6 0.0 0.0 0.0 5.299476040803846E-5 0.0 7 0.0 0.0 0.0 5.299476040803846E-5 0.0 8 0.0 0.0 0.0 5.299476040803846E-5 0.0 9 0.0 0.0 0.0 5.299476040803846E-5 0.0 10 0.0 0.0 0.0 5.299476040803846E-5 0.0 11 0.0 0.0 0.0 5.299476040803846E-5 0.0 12 0.0 0.0 0.0 5.299476040803846E-5 0.0 13 0.0 0.0 0.0 5.299476040803846E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 910 0.0 14.160291 4 TGTAGGA 2855 0.0 11.184629 2 TCGAGAC 60 0.005992085 11.054345 3 CTGTAGG 2690 0.0 11.0140085 1 GTAGGAC 2885 0.0 11.002344 3 TAGTACC 130 3.396417E-8 10.932577 4 AGGACCT 1705 0.0 10.891968 5 TAGAAAT 750 0.0 10.8645525 4 GGACGTG 2580 0.0 10.833704 6 TCGAACT 105 3.6278998E-6 10.816066 19 GTATAAG 195 5.456968E-12 10.815843 1 AGGACGT 2655 0.0 10.777476 5 CTAGGAC 255 0.0 10.775664 3 GACGTGG 1315 0.0 10.735819 7 GTCCTAA 1220 0.0 10.619536 1 GGCGAGG 1195 0.0 10.612416 19 CCAACGA 145 1.5312253E-8 10.443097 19 GGACCTG 1680 0.0 10.433671 6 GTCCTAC 2585 0.0 10.373526 1 GTCTTAT 205 1.2732926E-11 10.28824 1 >>END_MODULE