FastQCFastQC Report
Thu 26 May 2016
SRR1512557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886979
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT53420.28309801009974145No Hit
CTGTAGGACGTGGAATATGGCAAGA48760.25840245174959553No Hit
GTCCTAAAGTGTGTATTTCTCATTT46480.24631964637656273No Hit
GTATCAACGCAGAGTACTTTTTTTT42310.22422083128641074No Hit
CTTTAGGACGTGAAATATGGCGAGG42210.22369088368233034No Hit
GTCCTACAGTGTGCATTTCTCATTT31210.16539664723348801No Hit
TATCAACGCAGAGTACTTTTTTTTT26110.1383693194253884No Hit
GGTATCAACGCAGAGTACTTTTTTT25670.13603754996743472No Hit
CTGAAGGACCTGGAATATGGCGAGA21590.11441568772095503No Hit
GTACATGGGAAGCAGTGGTATCAAC21460.11372675583565053No Hit
CTGTAGGACCTGGAATATGGCGAGA20960.1110770178152486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8650.012.8523474
AGGACCT16650.011.1857745
GGCGAGG13350.010.81181819
TAGGCCC803.7643383E-410.68969254
GGACCTG16350.010.5754116
CTAGTAC1355.673246E-810.5574413
CGAAATC1355.6954377E-810.55464313
AATCCCG2550.010.42687719
TTAGGAC17900.010.4040933
TGGCGAG28050.010.3591718
TAAGACT1752.3464963E-1010.31640154
CTAGGAC2400.010.2935043
GACGTGA15650.010.2595317
GTCCTAA13800.010.2032121
GTCCTAC28550.010.1969331
AGGACGT27000.010.135685
GGACGTG26600.010.1076086
TTTAGGA19050.010.0752912
TAAGGCG856.620518E-410.05448916
TGTAGGA26950.010.012922
Produced by FastQC (version 0.11.5)