FastQCFastQC Report
Thu 26 May 2016
SRR1512556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872599
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68810.3674572078699177No Hit
GTCCTAAAGTGTGTATTTCTCATTT64960.3468975472057819No Hit
CTGTAGGACGTGGAATATGGCAAGA61930.330716827254527No Hit
CTTTAGGACGTGAAATATGGCGAGG52030.27784912840389214No Hit
GTATCAACGCAGAGTACTTTTTTTT36960.19737274237570349No Hit
GTCCTACAGTGTGCATTTCTCATTT34350.18343489449689976No Hit
CTGAAGGACCTGGAATATGGCGAGA28700.15326292495083038No Hit
GTCCTTCAGTGTGCATTTCTCATTT25510.1362277775434036No Hit
ATTTAGAAATGTCCACTGTAGGACG25270.13494613635914576No Hit
CTGTAGGACCTGGAATATGGCGAGA25060.13382470032292018No Hit
TTTCTAAATTTTCCACCTTTTTCAG23010.122877348540718No Hit
TATCAACGCAGAGTACTTTTTTTTT22460.11994025416012719No Hit
GGTATCAACGCAGAGTACTTTTTTT22370.11945963871603049No Hit
GAATATGGCAAGAAAACTGAAAATC21240.11342524480681662No Hit
GTACATGGGAAGCAGTGGTATCAAC20980.11203680019053733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG400.005376690514.21091655
TATACCG400.005376690514.21091655
CCAACGA1403.6379788E-1212.84473619
TAGGACC12200.012.7354664
CTGTGCG604.2593208E-412.6068799
TCCAACG2450.012.36019418
TGTCGAG1201.0608346E-811.82909118
TAGGACT4250.011.8145674
GGACGGG658.211741E-411.6602386
GGCGAGG18150.011.52431819
AGGACCG752.1278182E-411.3687335
GTCGAGG1105.215661E-711.185369519
AATGTCC9200.011.0944818
CACCTTT8250.011.030148514
CTAGGAC4050.010.9944533
AGGACCT24150.010.9450545
GTTTAGG2300.010.9211261
GGACCTG23350.010.8737346
ACCTTTT8350.010.7845315
AGGACGT37100.010.7762925
Produced by FastQC (version 0.11.5)