FastQCFastQC Report
Thu 26 May 2016
SRR1512556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872599
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73860.3944250744553426No Hit
CTGTAGGACGTGGAATATGGCAAGA72390.38657502220176343No Hit
GTCCTAAAGTGTGTATTTCTCATTT69000.3684718404741218No Hit
CTTTAGGACGTGAAATATGGCGAGG62070.3314644512786774No Hit
GTATCAACGCAGAGTACTTTTTTTT46230.2468761331176616No Hit
GTCCTACAGTGTGCATTTCTCATTT37970.20276631569278847No Hit
CTGAAGGACCTGGAATATGGCGAGA34300.18316788591684605No Hit
GGTATCAACGCAGAGTACTTTTTTT30550.1631422424128177No Hit
TATCAACGCAGAGTACTTTTTTTTT28860.15411735240700225No Hit
CTGTAGGACCTGGAATATGGCGAGA28100.15005882199018583No Hit
ATTTAGAAATGTCCACTGTAGGACG27830.1486169756578958No Hit
GTCCTTCAGTGTGCATTTCTCATTT25980.13873765819590847No Hit
TTTCTAAATTTTCCACCTTTTTCAG25950.13857745304787625No Hit
GAATATGGCAAGAAAACTGAAAATC23980.12805731499376No Hit
GTACATGGGAAGCAGTGGTATCAAC23620.12613485321737328No Hit
GGAATATGGCGAGAAAACTGAAAAT20960.11192999675851582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1550.014.7032918
CTATACG400.005267730414.254854
TAGGACC10450.013.3681844
ATAGGAC2550.013.0433053
GTCGAGG1457.2759576E-1212.4428719
GGCGAGG16200.011.48883619
ACCTTTT7950.011.22932615
TAAGACG600.00586155211.0871064
CACCTTT8250.010.93610314
CCACCTT8400.010.74138913
CCAACGT803.786619E-410.68285819
CCCGTTT1252.2464337E-710.63793612
TGTAGGA38100.010.5752122
GCCTTAC1451.4068064E-810.5000761
TGTCGAG1909.094947E-1210.4954418
AGGACCT23300.010.44165
CCTATAC1553.6434358E-910.4226243
TGGCGAG37800.010.35000118
GGACCTG23050.010.3041666
AATGTCC8300.010.3005828
Produced by FastQC (version 0.11.5)