FastQCFastQC Report
Thu 26 May 2016
SRR1512555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373349
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46770.19706330590233465No Hit
CTGTAGGACGTGGAATATGGCAAGA42640.179661735378994No Hit
GTATCAACGCAGAGTACTTTTTTTT42320.17831342967258504No Hit
GTCCTAAAGTGTGTATTTCTCATTT35210.14835576225831093No Hit
CTTTAGGACGTGAAATATGGCGAGG30330.12779410023557428No Hit
GGTATCAACGCAGAGTACTTTTTTT26710.11254139193182292No Hit
GTCCTACAGTGTGCATTTCTCATTT25000.10533638331320003No Hit
TATCAACGCAGAGTACTTTTTTTTT24340.10255550279373155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGA508.8617555E-515.17067217
TACCGTA456.905971E-414.7373897
CGGCGAG456.9560384E-414.723351518
TAGGACC9450.013.6345924
GTACCGT707.474666E-613.5343376
TACGGCG500.00152242213.27377516
GTAGGAC26250.011.5136983
TGTAGGA27700.011.4584972
GGACGTG22600.011.3185396
CTGTAGG26200.011.3033861
AGGACGT23050.011.2208765
GACGTGG12650.011.1591417
AGGACCT18150.011.0138935
GGCGAGG10200.010.85876519
TCCAACG2100.010.81715718
TAGGACG24900.010.6915834
GGACCTG18050.010.655016
GACGTGA11300.010.563977
ACACCGA909.786717E-510.5267076
GTCCTAC21150.010.48986151
Produced by FastQC (version 0.11.5)