FastQCFastQC Report
Thu 26 May 2016
SRR1512555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373349
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56000.23595349862156806No Hit
GTCCTACAGTGGACATTTCTAAATT48310.20355202711442774No Hit
CTGTAGGACGTGGAATATGGCAAGA47740.20115035757488678No Hit
CTTTAGGACGTGAAATATGGCGAGG36000.15168439197100805No Hit
GTCCTAAAGTGTGTATTTCTCATTT35930.1513894500977311No Hit
TATCAACGCAGAGTACTTTTTTTTT35290.14869283868491318No Hit
GGTATCAACGCAGAGTACTTTTTTT34980.1473866675318295No Hit
GTCCTACAGTGTGCATTTCTCATTT26650.11228858461187123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8600.013.0384274
ACCGGGC751.4806772E-512.665578
GGCGAGG9350.011.27353619
GTAGGAC23800.011.0597293
GTCCTAC26050.010.9959491
AATGCCG2250.010.9735918
TAGAAAT6700.010.9208784
AGGACGT22300.010.9090245
TGTAGGA25500.010.881162
GGACGTG22350.010.7975346
GACGTGG11650.010.6012827
AATGTCC5400.010.5546438
CTGTAGG24600.010.5222291
GATATAC6400.010.4085931
TCCTACA28350.010.3906132
GGTATCA14000.010.1961731
GACGTGA11400.010.1670737
GGACCTG15700.010.1666556
TGGCGAG22450.010.15202818
TAGGACG24450.010.1438624
Produced by FastQC (version 0.11.5)