FastQCFastQC Report
Thu 26 May 2016
SRR1512554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778400
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43130.24252136752136752No Hit
GTCCTACAGTGGACATTTCTAAATT35500.19961763382816017No Hit
CTGTAGGACGTGGAATATGGCAAGA31890.17931848852901486No Hit
GTCCTAAAGTGTGTATTTCTCATTT31310.17605713000449844No Hit
GGTATCAACGCAGAGTACTTTTTTT26440.14867296446243813No Hit
CTTTAGGACGTGAAATATGGCGAGG26000.14619883040935672No Hit
TATCAACGCAGAGTACTTTTTTTTT24680.13877642825011247No Hit
GTCCTACAGTGTGCATTTCTCATTT21160.11898335582546109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG500.001528749913.2661657
CAAGTCG1251.07320375E-1012.8864026
TAGGACC7300.012.7202614
AATCCCG658.3013036E-411.64510719
AGTCGTC1501.9281288E-1011.3405288
GGACCTG11750.011.2090746
AAGTCGT1553.274181E-1011.0041927
TGTAGGA21600.010.6606122
AGGACCT13000.010.6414865
GTATCAA20500.010.6268221
AGGACGT19750.010.6027365
GGACGTG19400.010.5498156
CTGTAGG19850.010.5196281
GGCGAGG8200.010.50012219
GTAGGAC20950.010.4935243
GTATAAG2400.010.4574621
GACGTGG9950.010.380567
GTCCTAC18800.010.1459631
ACAGACG750.00276527210.0804698
TCGTCAA1606.4737833E-910.06437110
Produced by FastQC (version 0.11.5)