FastQCFastQC Report
Thu 26 May 2016
SRR1512554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778400
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52980.29790823211875844No Hit
CTGTAGGACGTGGAATATGGCAAGA36280.20400359874044086No Hit
GTCCTACAGTGGACATTTCTAAATT36220.20366621682411157No Hit
GGTATCAACGCAGAGTACTTTTTTT34510.19405083220872696No Hit
TATCAACGCAGAGTACTTTTTTTTT34410.1934885290148448No Hit
GTCCTAAAGTGTGTATTTCTCATTT31230.17560728744939272No Hit
CTTTAGGACGTGAAATATGGCGAGG30210.16987179487179488No Hit
GTCCTACAGTGTGCATTTCTCATTT21670.12185110211426001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7400.011.6863094
CTAGAGC1251.8229912E-811.4037823
TGCGCCA855.326997E-511.175776512
GGACCGT600.00587279211.0842036
GTCCTAT1408.141797E-910.8748231
TTAGGAC11750.010.8376373
AGGACGT19950.010.8134145
AATGTCC5800.010.8096728
GGTATCA13300.010.8032781
GTATTAG1855.456968E-1210.8013441
GGACGTG20200.010.7706476
GTCCTAC19100.010.5118531
GGACCTG14350.010.4607526
TTTAGGA12400.010.4228122
AAATGTC5750.010.4083417
GACGTGA11150.010.3090667
AGGACCT14900.010.2687965
GACGTGG10000.010.2596497
GTCCTAA9800.010.1951461
ACTGTGC3650.010.1500548
Produced by FastQC (version 0.11.5)