FastQCFastQC Report
Thu 26 May 2016
SRR1512553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891779
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT53230.28137536149835685No Hit
GTCCTAAAGTGTGTATTTCTCATTT49400.26112986770653446No Hit
CTGTAGGACGTGGAATATGGCAAGA45770.24194157985684372No Hit
GTATCAACGCAGAGTACTTTTTTTT40390.21350274001350053No Hit
CTTTAGGACGTGAAATATGGCGAGG40100.21196979139740954No Hit
GTCCTACAGTGTGCATTTCTCATTT31330.16561131083493366No Hit
GGTATCAACGCAGAGTACTTTTTTT25260.13352511049123603No Hit
TATCAACGCAGAGTACTTTTTTTTT23880.12623038949052717No Hit
CTGTAGGACCTGGAATATGGCGAGA22460.11872422730139197No Hit
CTGAAGGACCTGGAATATGGCGAGA21520.1137553593733729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT400.00546917214.1744378
TAGGACC9950.013.1408424
TCCAACG2450.012.7459718
GTACCGT604.1856922E-412.6329846
CCAACGA1700.012.2487319
AGGACCT18850.011.6612175
GGACCTG18800.011.3394476
GGCGAGG11550.011.30874419
TGTGCGA600.00601898711.04769510
TAGAAAT7400.011.0111834
TGTAGGA33200.010.98697952
TGGCGAG29150.010.90751418
GTCCTAC28050.010.8828631
GATATAC7050.010.8249761
TGCCGGT2050.010.62426510
ATTATAC2150.010.5764523
GTAGGAC32450.010.5696623
TTAGGAC17500.010.5575663
GGTATCA10850.010.5506111
AGGACGT29750.010.5416415
Produced by FastQC (version 0.11.5)