FastQCFastQC Report
Thu 26 May 2016
SRR1512553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891779
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57260.30267806123231095No Hit
CTGTAGGACGTGGAATATGGCAAGA52780.27899664812856045No Hit
GTCCTAAAGTGTGTATTTCTCATTT50620.2675788239535379No Hit
GTATCAACGCAGAGTACTTTTTTTT48240.2549980732421705No Hit
CTTTAGGACGTGAAATATGGCGAGG45900.24262876371922937No Hit
GGTATCAACGCAGAGTACTTTTTTT33040.17465042164015987No Hit
GTCCTACAGTGTGCATTTCTCATTT32800.1733817745096018No Hit
TATCAACGCAGAGTACTTTTTTTTT32630.17248314945878984No Hit
CTGAAGGACCTGGAATATGGCGAGA25410.13431801494783482No Hit
CTGTAGGACCTGGAATATGGCGAGA25370.1341065737594085No Hit
ATTTAGAAATGTCCACTGTAGGACG20550.10862791055403406No Hit
TTTCTAAATTTTCCACCTTTTTCAG20220.10688352074951672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9150.012.8771844
GGTATCA13050.011.7340891
CGGTTTC1701.8189894E-1211.73356313
CCAACGA1552.5465852E-1111.64098119
ACCTTTT7800.011.56727715
TCCAACG2550.011.545485518
ATAGGAC2800.011.5382833
AGGACCT17800.011.4775795
GGACGTA855.3205706E-511.1771866
AATCCCG3750.011.14266619
GGGCGAA600.005878285511.082870511
GTCCTAT2700.010.9202351
TGTCCAC8050.010.856976510
GGCGAGG12000.010.84194119
GGACCTG17900.010.8275086
CCGGTTT1855.456968E-1210.782763512
CACCTTT8300.010.75602714
CCACCTT8100.010.67126613
AGGCGTG909.515586E-510.5556737
GCCGGTT1909.094947E-1210.49956211
Produced by FastQC (version 0.11.5)