FastQCFastQC Report
Thu 26 May 2016
SRR1512552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526147
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC47990.3144520154349483No Hit
CCCATGTACTCTGCGTTGATACCAC35090.22992542658079465No Hit
GTATCAACGCAGAGTACTTTTTTTT32930.2157721372842852No Hit
GTCCTACAGTGGACATTTCTAAATT30780.2016843724752596No Hit
CTGTAGGACGTGGAATATGGCAAGA28350.18576192201668645No Hit
GTCCTAAAGTGTGTATTTCTCATTT26870.17606429786907815No Hit
CTTTAGGACGTGAAATATGGCGAGG24550.16086261677282726No Hit
GAGTACATGGGAAGCAGTGGTATCA21470.14068107462780452No Hit
GGTATCAACGCAGAGTACTTTTTTT19360.1268554077687143No Hit
TATCAACGCAGAGTACTTTTTTTTT19040.12475862416923142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCG307.862748E-418.9492575
TCCAACG1203.6379788E-1214.19463718
TAGGACC4750.012.7658164
CCGGTTT1052.0767402E-812.62950912
CCAACGA854.1169824E-612.24675719
CGGTTTC951.0708427E-611.97071413
GCCGGTT1157.369454E-811.53091111
GTCTTAC1251.04711944E-711.2420491
TGCCGGT1303.4122422E-810.92866310
CCCTTAG1207.5037497E-710.8740061
GTCCTAC16400.010.8332021
GTCCTAA9750.010.7067131
GTCCTAG1801.4370016E-1010.5951841
TCTAGGA1701.4551915E-1010.589642
TATGGAC909.771971E-510.5277123
GTTAGAC909.771971E-510.5277123
GGCGAGG7600.010.4595519
GGACCTG9550.010.4174746
ATTTCTA12950.010.39533415
CTTATAC1553.8216967E-910.39187053
Produced by FastQC (version 0.11.5)