FastQCFastQC Report
Thu 26 May 2016
SRR1512552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526147
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC53890.3531114630504139No Hit
CCCATGTACTCTGCGTTGATACCAC41150.2696332659960017No Hit
GTATCAACGCAGAGTACTTTTTTTT41080.2691745945836148No Hit
GTCCTACAGTGGACATTTCTAAATT34090.22337297783241064No Hit
CTGTAGGACGTGGAATATGGCAAGA31000.20312591119990409No Hit
GTCCTAAAGTGTGTATTTCTCATTT29270.19179017486519975No Hit
GGTATCAACGCAGAGTACTTTTTTT27220.17835765493101255No Hit
CTTTAGGACGTGAAATATGGCGAGG27040.17717821415630342No Hit
TATCAACGCAGAGTACTTTTTTTTT26110.1710844368203063No Hit
GAGTACATGGGAAGCAGTGGTATCA25290.1657114288466314No Hit
GTCCTACAGTGTGCATTTCTCATTT17120.11217792257233411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT456.756306E-414.7794866
CTGATCG400.00528073614.2493129
GGTCTAC1457.2759576E-1212.4652431
TAGGACC4850.011.7558634
GTCCTAG2100.011.3249391
CTATACT1354.7057256E-911.2624064
ATCGTGC600.00587768911.0827978
TCCAACG951.3662146E-510.99550418
TAGGACT1855.456968E-1210.7867994
GGCGAGG8550.010.77337319
GGACGTG18450.010.7627616
AGGACGT18850.010.5371375
GACGTGA10450.010.3644947
TTAGGAC11750.010.2709563
GTATTAA2600.010.2447151
AATGTCC5050.010.1579268
TGGCGAG17550.010.06426118
GGTTTAA951.6213361E-410.0136311
GTACTAG951.6213361E-410.0136311
ACGTGAA12050.010.0119658
Produced by FastQC (version 0.11.5)