FastQCFastQC Report
Thu 26 May 2016
SRR1512551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622595
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39050.24066387484245913No Hit
GTCCTAAAGTGTGTATTTCTCATTT36360.22408549268301703No Hit
GTATCAACGCAGAGTACTTTTTTTT36220.22322267725464456No Hit
CTGTAGGACGTGGAATATGGCAAGA34710.21391659656291312No Hit
CTTTAGGACGTGAAATATGGCGAGG32510.20035806840277454No Hit
GGTATCAACGCAGAGTACTTTTTTT23280.14347387980364784No Hit
TATCAACGCAGAGTACTTTTTTTTT22770.14033076645743392No Hit
GTCCTACAGTGTGCATTTCTCATTT22330.1376190608254062No Hit
CTGAAGGACCTGGAATATGGCGAGA16370.10088777544612179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA854.107471E-612.249413519
TAAGACG701.11893685E-412.1811834
GTCCTAT1900.012.1390451
TCCAACG1302.7739588E-911.64834918
TAGGACC6050.011.4317444
GGCGAGG10400.011.28573619
GTCTAAG1251.06132575E-711.2312561
GTATAAG1705.0931703E-1111.2076611
GGACGGG1105.141246E-711.1975386
GTCGAGG1105.20753E-711.18644719
GTCCTAC19100.011.077951
AAGACGT600.005995702511.0532955
GTGTAGG3200.010.9680241
GTGCGCC951.4055549E-510.9674711
TGCGCCA1053.5843877E-610.82671212
AAGTCGT1252.3219036E-710.6121517
TGTCGAG1252.3588291E-710.599997518
TGTAGGA21850.010.536912
ATCCCGT909.807722E-510.524013510
GCGTGCG909.978392E-510.5061169
Produced by FastQC (version 0.11.5)