FastQCFastQC Report
Thu 26 May 2016
SRR1512551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622595
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43590.2686437465911087No Hit
GTCCTACAGTGGACATTTCTAAATT40560.2499699555341906No Hit
CTGTAGGACGTGGAATATGGCAAGA40470.24941528847309402No Hit
GTCCTAAAGTGTGTATTTCTCATTT38100.23480905586421752No Hit
CTTTAGGACGTGAAATATGGCGAGG36100.22248312117318247No Hit
TATCAACGCAGAGTACTTTTTTTTT29090.17928072008110466No Hit
GGTATCAACGCAGAGTACTTTTTTT27800.17133049220538704No Hit
GTCCTACAGTGTGCATTTCTCATTT24480.1508694406182689No Hit
CTGAAGGACCTGGAATATGGCGAGA19540.12042438193141233No Hit
CTGTAGGACCTGGAATATGGCGAGA16980.10464718552688748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG500.001496153213.3040585
TAGGACC6800.013.27529054
AGCACCG701.0878541E-412.2180135
GACCGGG550.003067202612.0916157
TCCAACG1406.8394E-1011.5314118
GGACCGG752.0710156E-411.4017216
GGACCTG12650.011.1914126
GGCGAGG10050.011.14972419
GCGTGCG600.005878683611.0826149
CGTGCGC600.00588003311.08227310
CACCTTT5550.010.9518814
GTCCTAA11950.010.908331
TGTAGGA24150.010.8597862
AATGGCG700.001496112910.85309216
AGGACCT13700.010.7514545
GTATAGG1605.293259E-1010.7042951
GCACCGT803.7656107E-410.6891136
GACGTGG12050.010.6437757
AGGACGT23250.010.5451525
CCACCTT5700.010.49802413
Produced by FastQC (version 0.11.5)