FastQCFastQC Report
Thu 26 May 2016
SRR1512550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504014
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35720.2374977892493022No Hit
GTACATGGGAAGCAGTGGTATCAAC34090.22666012417437603No Hit
CCCATGTACTCTGCGTTGATACCAC26140.1738015736555644No Hit
TATCAACGCAGAGTACTTTTTTTTT22550.14993211499361045No Hit
GGTATCAACGCAGAGTACTTTTTTT22460.14933371630849182No Hit
GTCCTACAGTGGACATTTCTAAATT17120.11382872765812019No Hit
CTGTAGGACGTGGAATATGGCAAGA15740.10465328115296799No Hit
GTCCTAAAGTGTGTATTTCTCATTT15500.10305755132598499No Hit
GAGTACATGGGAAGCAGTGGTATCA15270.10152831024179297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA350.002214448816.23651510
CAAGACG552.0093133E-413.777824
TACACCG500.001532919513.2611495
GCCCTAG1006.929804E-813.0633841
GCACCGT701.121042E-412.1786086
TAGGACC3200.012.136324
ACACCGT550.00313630912.0555926
GTATAAC1102.3993016E-711.8758051
AGGACGT9800.011.5986735
GGACGTG9400.011.4876216
GACGTGA5250.011.36677
TAGGACG10150.011.2920424
TTAGGAC6250.011.0639583
GTCCTAA6100.011.037161
GTCTAGA1653.4924597E-1010.9622811
GTGTAGG2051.8189894E-1210.7840331
GTATTAA2150.010.7498341
AGCACCG1159.1520997E-710.7077615
GTAGGAC8300.010.613843
CTAGGAC2450.010.4391553
Produced by FastQC (version 0.11.5)