FastQCFastQC Report
Thu 26 May 2016
SRR1512550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504014
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45540.302789734670023No Hit
GTACATGGGAAGCAGTGGTATCAAC39280.26116778168288324No Hit
GGTATCAACGCAGAGTACTTTTTTT30950.2057826589380152No Hit
TATCAACGCAGAGTACTTTTTTTTT30860.2051842602528966No Hit
CCCATGTACTCTGCGTTGATACCAC30290.20139440191381197No Hit
GTCCTACAGTGGACATTTCTAAATT18230.12120897810791655No Hit
CTGTAGGACGTGGAATATGGCAAGA17800.11834996216790535No Hit
GAGTACATGGGAAGCAGTGGTATCA17500.1163552998841766No Hit
GTCCTAAAGTGTGTATTTCTCATTT16460.10944047063391696No Hit
CTTTAGGACGTGAAATATGGCGAGG15090.10033151287155571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGG550.003068083712.0910576
GTACTAG855.251784E-511.1911261
TAGGACC3750.010.8949174
GCACCGG700.001491200810.8572766
CGTGCGC803.7724117E-410.6869210
GTCCTAA4900.010.6772471
TGGACCG909.4964664E-510.5574415
ATTATAC1451.4391844E-810.48428253
AGCACCG1002.3935067E-510.4518665
TTAGACT1106.033155E-610.3651424
TGTAGGA11450.010.2897042
GTCGTGG750.002618859310.1466211
AGGGCCG750.002643565910.1351425
AACCGTG750.00264794710.1331217
TTAACCT856.5838755E-410.0602864
CTACACT2002.5465852E-119.976454
AGGCGTG1252.5694808E-69.8797927
GACGTGA5300.09.8582837
GTCATAG1451.5854494E-79.8404721
CTGTAGG10650.09.6464351
Produced by FastQC (version 0.11.5)