FastQCFastQC Report
Thu 26 May 2016
SRR1512549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42264
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC18294.327560098428923No Hit
CCCATGTACTCTGCGTTGATACCAC14503.4308158243422295No Hit
GAGTACATGGGAAGCAGTGGTATCA8512.0135339769070604No Hit
GTATCAACGCAGAGTACTTTTTTTT7871.8621048646602307No Hit
GGTATCAACGCAGAGTACTTTTTTT5441.2871474540980503No Hit
CATGTACTCTGCGTTGATACCACTG5301.2540223357940563No Hit
TATCAACGCAGAGTACTTTTTTTTT5171.2232632973689193No Hit
ACGCAGAGTACATGGGAAGCAGTGG4861.1499148211243613No Hit
TATCAACGCAGAGTACATGGGAAGC4801.135718341851221No Hit
GCGTTGATACCACTGCTTCCCATGT4621.0931289040318002No Hit
GGTATCAACGCAGAGTACATGGGAA4511.0671020253643764No Hit
GTATCAACGCAGAGTACATGGGAAG4281.012682188150672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4190.9913874692409615No Hit
ACTCTGCGTTGATACCACTGCTTCC3930.9298693923906871No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3670.8683513155404126No Hit
GTGGTATCAACGCAGAGTACATGGG2900.686163164868446No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2560.6057164489873178No Hit
GTACTCTGCGTTGATACCACTGCTT2400.5678591709256104No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2220.5252697331061896No Hit
GGGAAGCAGTGGTATCAACGCAGAG2190.5181714934696195No Hit
GCTTCCCATGTACTCTGCGTTGATA2120.5016089343176225No Hit
ATACCACTGCTTCCCATGTACTCTG2030.4803142154079122No Hit
CAGTGGTATCAACGCAGAGTACATG1860.4400908574673481No Hit
ACATGGGAAGCAGTGGTATCAACGC1810.42826045807306456No Hit
GCAGAGTACATGGGAAGCAGTGGTA1750.4140639787999243No Hit
GTTGATACCACTGCTTCCCATGTAC1670.3951353397690706No Hit
GCAGTGGTATCAACGCAGAGTACAT1650.3904031800113572No Hit
CATGGGAAGCAGTGGTATCAACGCA1650.3904031800113572No Hit
GATACCACTGCTTCCCATGTACTCT1650.3904031800113572No Hit
TACCACTGCTTCCCATGTACTCTGC1640.3880371001325005No Hit
AAGCAGTGGTATCAACGCAGAGTAC1590.37620670073821694No Hit
ATGGGAAGCAGTGGTATCAACGCAG1460.34544766231307966No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1440.34071550255536626No Hit
CTGCTTCCCATGTACTCTGCGTTGA1380.326519023282226No Hit
CCACTGCTTCCCATGTACTCTGCGT1250.29575998485708876No Hit
NTACATGGGAAGCAGTGGTATCAAC1210.28629566534166195No Hit
CTTCCCATGTACTCTGCGTTGATAC1180.27919742570509176No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1170.2768313458262351No Hit
ACCATGTACTCTGCGTTGATACCAC1100.26026878667423814No Hit
TTCCCATGTACTCTGCGTTGATACC1070.253170547037668No Hit
CCATGTACTCTGCGTTGATACCACT1050.2484383872799546No Hit
ATCAACGCAGAGTACATGGGAAGCA1010.2389740677645277No Hit
GTGGTATCAACGCAGAGTACTTTTT960.2271436683702442No Hit
NCCATGTACTCTGCGTTGATACCAC900.21294718909710392No Hit
TGATACCACTGCTTCCCATGTACTC890.21058110921824721No Hit
GTACATGGGGAGGAAAAAAAAAAAA880.2082150293393905No Hit
GGAAGCAGTGGTATCAACGCAGAGT860.20348286958167708No Hit
GTACATGGTAAGCAGTGGTATCAAC790.1869203104296801No Hit
ATCAACGCAGAGTACTTTTTTTTTT700.16562559151996972No Hit
NAGTACATGGGAAGCAGTGGTATCA670.1585273518833996No Hit
GTACATGGGGAAAAAAAAAAAAAAA660.15616127200454286No Hit
NTATCAACGCAGAGTACTTTTTTTT620.14669695248911602No Hit
GGTATCAACGCAGAGTACATGGGGA620.14669695248911602No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT590.13959871285254588No Hit
TGGGAAGCAGTGGTATCAACGCAGA550.13013439333711907No Hit
AAAAAGTACTCTGCGTTGATACCAC550.13013439333711907No Hit
CTGCGTTGATACCACTGCTTCCCAT510.12067007382169223No Hit
GCCATGTACTCTGCGTTGATACCAC510.12067007382169223No Hit
GGTATCAACGCAGAGTACATGGGGG500.11830399394283551No Hit
GGTAAGCAGTGGTATCAACGCAGAG470.11120575430626538No Hit
GTACTGGTTCACTATCGGTCAGTCA460.10883967442740868No Hit
CTCTGCGTTGATACCACTGCTTCCC450.10647359454855196No Hit
NGTATCAACGCAGAGTACTTTTTTT450.10647359454855196No Hit
AGTGGTATCAACGCAGAGTACATGG430.10174143479083854No Hit
GTACATGGGTAAGCAGTGGTATCAA430.10174143479083854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT6100.06.522606413
AGTGGTA6150.06.469577314
TGCGTTG4700.06.449912512
CCATGTA4700.06.44991252
AAGCAGT6200.06.41740310
AGCAGTG6250.06.36606411
GCAGTGG6250.06.36606412
CTGCGTT4851.8189894E-126.25043111
CTCTGCG4851.8189894E-126.24302539
GAAGCAG5650.06.19640979
TCTGCGT4903.6379788E-126.18665110
CCCATGT4809.640644E-116.07666871
GCGTTGA5057.2759576E-126.002889613
ACTCTGC5057.2759576E-125.99577678
CGTTGAT4901.8189894E-115.993318614
GGAAGCA5850.05.98456678
TACTCTG5109.094947E-125.94403747
TTGATAC4807.094059E-115.92081916
TGATACC4901.2187229E-105.799985417
TGTACTC5252.0008883E-115.77420765
Produced by FastQC (version 0.11.5)