FastQCFastQC Report
Thu 26 May 2016
SRR1512549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42264
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC21675.127295097482492No Hit
CCCATGTACTCTGCGTTGATACCAC14373.4000567859170925No Hit
GAGTACATGGGAAGCAGTGGTATCA10352.4488926746166952No Hit
GTATCAACGCAGAGTACTTTTTTTT9342.2099186068521672No Hit
GGTATCAACGCAGAGTACTTTTTTT7371.7438008707173953No Hit
TATCAACGCAGAGTACTTTTTTTTT7291.7248722316865417No Hit
CATGTACTCTGCGTTGATACCACTG6191.4646034450123036No Hit
ACGCAGAGTACATGGGAAGCAGTGG6001.4196479273140261No Hit
TATCAACGCAGAGTACATGGGAAGC5961.4101836077985992No Hit
GGTATCAACGCAGAGTACATGGGAA5401.2776831345826234No Hit
GTATCAACGCAGAGTACATGGGAAG5191.2279954571266325No Hit
GCGTTGATACCACTGCTTCCCATGT5001.183039939428355No Hit
CTTATACACATCTCCGAGCCCACGA4220.9984857088775316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4000.9464319515426841No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3940.9322354722695438No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3860.9133068332386902No Hit
GTGGTATCAACGCAGAGTACATGGG3750.8872799545712663No Hit
ACTCTGCGTTGATACCACTGCTTCC3740.8849138746924097No Hit
ATACACATCTCCGAGCCCACGAGAC2910.6885292447473027No Hit
GGGAAGCAGTGGTATCAACGCAGAG2710.6412076471701684No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2540.6009842892296045No Hit
GCAGTGGTATCAACGCAGAGTACAT2520.596252129471891No Hit
CAGTGGTATCAACGCAGAGTACATG2470.5844217300776073No Hit
CATGGGAAGCAGTGGTATCAACGCA2380.5631270111678971No Hit
GTACTCTGCGTTGATACCACTGCTT2180.5158054135907628No Hit
GCTTCCCATGTACTCTGCGTTGATA2160.5110732538330494No Hit
GCAGAGTACATGGGAAGCAGTGGTA2150.5087071739541927No Hit
ACATGGGAAGCAGTGGTATCAACGC2070.48977853492333906No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2000.47321597577134206No Hit
ATACCACTGCTTCCCATGTACTCTG1990.47084989589248527No Hit
GATACCACTGCTTCCCATGTACTCT1980.46848381601362865No Hit
ACACATCTCCGAGCCCACGAGACCT1930.4566534166193451No Hit
AAGCAGTGGTATCAACGCAGAGTAC1850.4377247775884914No Hit
ATGGGAAGCAGTGGTATCAACGCAG1830.43299261783077797No Hit
TACCACTGCTTCCCATGTACTCTGC1570.37147454098050353No Hit
CTGCTTCCCATGTACTCTGCGTTGA1540.3643763013439334No Hit
CCACTGCTTCCCATGTACTCTGCGT1420.33598334279765285No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1350.31942078364565585No Hit
GTGGTATCAACGCAGAGTACTTTTT1260.2981260647359455No Hit
GTTGATACCACTGCTTCCCATGTAC1250.29575998485708876No Hit
TGATACCACTGCTTCCCATGTACTC1240.29339390497823203No Hit
ATCAACGCAGAGTACATGGGAAGCA1140.26973310618966495No Hit
CCATGTACTCTGCGTTGATACCACT1050.2484383872799546No Hit
ATCAACGCAGAGTACTTTTTTTTTT1020.24134014764338446No Hit
CTTCCCATGTACTCTGCGTTGATAC1020.24134014764338446No Hit
GGAAGCAGTGGTATCAACGCAGAGT1010.2389740677645277No Hit
TTCCCATGTACTCTGCGTTGATACC990.23424190800681433No Hit
GGTATCAACGCAGAGTACATGGGGA790.1869203104296801No Hit
CATCTCCGAGCCCACGAGACCTCTC760.17982207079310997No Hit
GTACATGGGGAGGAAAAAAAAAAAA750.17745599091425326No Hit
GGTATCAACGCAGAGTACATGGGGG750.17745599091425326No Hit
GTACATGGTAAGCAGTGGTATCAAC710.16799167139882643No Hit
TGGGAAGCAGTGGTATCAACGCAGA650.15379519212568615No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT640.15142911224682945No Hit
GTACATGGGTAAGCAGTGGTATCAA620.14669695248911602No Hit
AAAAAGTACTCTGCGTTGATACCAC610.14433087261025931No Hit
ACCATGTACTCTGCGTTGATACCAC570.13486655309483248No Hit
GGTAAGCAGTGGTATCAACGCAGAG570.13486655309483248No Hit
GTACATGGGGAAAAAAAAAAAAAAA550.13013439333711907No Hit
AGTGGTATCAACGCAGAGTACATGG550.13013439333711907No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT530.12540223357940564No Hit
AACGCAGAGTACTTTTTTTTTTTTT510.12067007382169223No Hit
CACATCTCCGAGCCCACGAGACCTC510.12067007382169223No Hit
TCTCCGAGCCCACGAGACCTCTCTA500.11830399394283551No Hit
TCAACGCAGAGTACATGGGAAGCAG490.11593791406397881No Hit
AAAGTACTCTGCGTTGATACCACTG480.1135718341851221No Hit
AACGCAGAGTACATGGGAAGCAGTG450.10647359454855196No Hit
TCAACGCAGAGTACTTTTTTTTTTT430.10174143479083854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC451.5915266E-619.01
TTATACA607.176095E-817.4166662
TATACAC555.5819146E-717.2727283
GAGACCT551.8901342E-413.81818219
ATACACA751.39977765E-512.6666674
CGAGACC603.947657E-412.66666618
TACACAT855.0446117E-511.176475
GAAGCAG5150.09.2233019
GGAAGCA5350.09.0560758
CAGTGGT5900.08.37288213
AAGCAGT5900.08.37288210
CACATCT1150.00105573758.2608697
ACATCTC1150.00105573758.2608698
ACACATC1150.00105573758.2608696
AGCAGTG6000.08.23333411
GGGAAGC6000.08.2333347
AGTGGTA6050.08.16528914
ATCTCCG1200.00160820157.91666610
CATCTCC1200.00160820157.9166669
GCAGTGG6450.07.658914612
Produced by FastQC (version 0.11.5)