##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42264 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.6920547037668 31.0 31.0 34.0 28.0 34.0 2 30.89331345826235 31.0 31.0 34.0 27.0 34.0 3 31.02181525648306 33.0 31.0 34.0 27.0 34.0 4 34.5987128525459 37.0 35.0 37.0 32.0 37.0 5 33.905096536059055 35.0 35.0 37.0 30.0 37.0 6 34.281421540791214 37.0 35.0 37.0 31.0 37.0 7 34.21772667045239 37.0 35.0 37.0 30.0 37.0 8 34.25695627484384 37.0 35.0 37.0 30.0 37.0 9 35.684696195343555 39.0 37.0 39.0 30.0 39.0 10 35.603492333901194 39.0 37.0 39.0 30.0 39.0 11 35.674616695059626 39.0 37.0 39.0 30.0 39.0 12 35.54538141207647 39.0 37.0 39.0 30.0 39.0 13 35.447898921067576 39.0 37.0 39.0 30.0 39.0 14 36.43062653795192 40.0 37.0 41.0 29.0 41.0 15 36.293701495362484 40.0 36.0 41.0 28.0 41.0 16 36.04322827938671 40.0 36.0 41.0 27.0 41.0 17 36.31485424947946 40.0 37.0 41.0 30.0 41.0 18 36.26057637705849 40.0 37.0 41.0 29.0 41.0 19 36.16283361726292 40.0 36.0 41.0 28.0 41.0 20 36.33238690138179 40.0 37.0 41.0 29.0 41.0 21 36.25863619155783 40.0 37.0 41.0 29.0 41.0 22 36.316013628620105 40.0 37.0 41.0 30.0 41.0 23 36.217087828885106 40.0 36.0 41.0 30.0 41.0 24 36.16023092939618 40.0 36.0 41.0 30.0 41.0 25 36.09629945106947 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.689468903021325 1101 2 -2.1660878785352082 1101 3 -1.5144788962208224 1101 4 -1.9878314889958872 1101 5 -2.2045591294439824 1101 6 -1.8953304821058694 1101 7 -1.584179279694105 1101 8 -1.1126187573523225 1101 9 -0.5306247465761018 1101 10 -1.5811692659221208 1101 11 -1.9455656320068186 1101 12 -1.442199292357806 1101 13 -1.103045566091609 1101 14 -1.0425835054415273 1101 15 -0.5014612694633058 1101 16 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-0.3133997064921985 2316 3 -0.1682423370810362 2316 4 -0.671702456737826 2316 5 -0.41853762406763906 2316 6 -1.4200616649015672 2316 7 -1.2492330431349643 2316 8 -2.02713488638458 2316 9 -2.2730978648556714 2316 10 -2.8800939971049146 2316 11 -2.5396516535121947 2316 12 -2.4233820880567265 2316 13 -1.7412176091023568 2316 14 -1.380755548452278 2316 15 -0.9944720221514771 2316 16 -1.0353923384251686 2316 17 -1.5684230715714875 2316 18 -2.1556091158004946 2316 19 -1.9893782210581747 2316 20 -2.0949678775409737 2316 21 -1.6373586304886487 2316 22 -1.8009880689383593 2316 23 -1.8983762841991876 2316 24 -3.366040856630569 2316 25 -2.57299470323985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 814.0 3 0.0 4 9.0 5 129.0 6 174.0 7 109.0 8 94.0 9 84.0 10 83.0 11 87.0 12 71.0 13 66.0 14 72.0 15 59.0 16 54.0 17 48.0 18 66.0 19 65.0 20 106.0 21 120.0 22 151.0 23 191.0 24 208.0 25 257.0 26 291.0 27 299.0 28 380.0 29 419.0 30 617.0 31 695.0 32 930.0 33 1262.0 34 1666.0 35 2258.0 36 3822.0 37 8247.0 38 18261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.686402729405074 19.636553178382734 12.675622527068969 20.001421565143225 2 14.245149077141505 24.54803596781827 36.45054424988168 24.756270705158542 3 22.040034071550256 32.6140450501609 28.610637895135337 16.73528298315351 4 13.264243800870718 20.499716070414536 29.822070793109972 36.41396933560477 5 10.579130475942538 42.28102146593141 32.88050552623482 14.259342531891225 6 29.464666067117907 31.89757182751929 19.236048658115205 19.401713447247598 7 22.65079628008235 35.89057952152204 21.25701034099245 20.201613857403157 8 30.597950829369868 29.798159059179856 22.136248550673198 17.467641560777075 9 29.945575011831522 13.584950307619497 19.219119734973972 37.25035494557501 10 20.234264079507806 31.327496450544253 27.991008045433034 20.447231424514907 11 28.03388867853086 26.817493373722073 18.03530859522908 27.113309352517984 12 26.058971082398596 32.50035496237399 25.621184154479625 15.819489800747789 13 31.800757217226693 21.062470421202082 23.932796971131093 23.20397539044013 14 23.629350938217268 28.146518066302264 25.687986559712268 22.5361444357682 15 34.0171789593242 22.062894867608435 23.68614088639644 20.233785286670926 16 18.44209928541006 28.020443897591218 32.130519142492076 21.406937674506647 17 24.170077845869912 30.87807301895275 27.011807017958972 17.940042117218372 18 23.606937510351845 22.45462934481698 32.96027257885148 20.9781605659797 19 21.472174900624644 28.930058678780995 34.32235472269544 15.275411697898921 20 23.509369676320272 23.895040696573915 31.246450880181715 21.349138746924094 21 20.17319704713231 26.61839863713799 34.65833806549309 18.550066250236608 22 20.620874050587982 28.689397345195562 27.331235359534347 23.35849324468211 23 17.658054135907626 27.479651713041832 32.51940185500663 22.342892296043914 24 20.86458604453068 32.84433192153893 28.424390128481175 17.866691905449212 25 20.315161839863713 25.321786863524515 27.314026121521863 27.049025175089913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 623.0 1 623.0 2 412.5 3 202.0 4 202.0 5 202.0 6 336.5 7 471.0 8 471.0 9 471.0 10 406.5 11 342.0 12 342.0 13 342.0 14 245.5 15 149.0 16 149.0 17 149.0 18 294.0 19 439.0 20 439.0 21 439.0 22 736.0 23 1033.0 24 1033.0 25 1033.0 26 1438.0 27 1843.0 28 1843.0 29 1843.0 30 2060.5 31 2278.0 32 2278.0 33 2278.0 34 2290.0 35 2302.0 36 2302.0 37 2302.0 38 2302.0 39 2302.0 40 2302.0 41 2302.0 42 2507.0 43 2712.0 44 2712.0 45 2712.0 46 6926.5 47 11141.0 48 11141.0 49 11141.0 50 9622.5 51 8104.0 52 8104.0 53 8104.0 54 5985.5 55 3867.0 56 3867.0 57 3867.0 58 2892.0 59 1917.0 60 1917.0 61 1917.0 62 1614.5 63 1312.0 64 1312.0 65 1312.0 66 1006.5 67 701.0 68 701.0 69 701.0 70 510.0 71 319.0 72 319.0 73 319.0 74 225.5 75 132.0 76 132.0 77 132.0 78 90.5 79 49.0 80 49.0 81 49.0 82 34.5 83 20.0 84 20.0 85 20.0 86 11.0 87 2.0 88 2.0 89 2.0 90 2.0 91 2.0 92 2.0 93 2.0 94 2.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13486655309483248 2 0.00946431951542684 3 0.0 4 0.0 5 0.026026878667423814 6 0.023660798788567104 7 0.011830399394283552 8 0.007098239636570131 9 0.00946431951542684 10 0.00946431951542684 11 0.01892863903085368 12 0.014196479273140262 13 0.00946431951542684 14 0.007098239636570131 15 0.007098239636570131 16 0.00473215975771342 17 0.00236607987885671 18 0.00236607987885671 19 0.0 20 0.0 21 0.0 22 0.00236607987885671 23 0.0 24 0.00236607987885671 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 42264.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.746924096157485 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28651685393258 32.27096346772667 2 10.014655593551538 7.760742002650009 3 2.7784562774792376 3.229699034639409 4 1.2579384465070835 1.9496498201779293 5 0.5068392769907181 0.9819231497255347 6 0.36028334147532975 0.8375922771152754 7 0.201514411333659 0.5465644520159001 8 0.15266243282852954 0.47321597577134206 9 0.134342940889106 0.46848381601362865 >10 0.9220810942843185 6.7054703766799175 >50 0.1038104543234001 2.6168843460155213 >100 0.21372740595994139 18.703861442362292 >500 0.048851978505129456 12.47870528109029 >1k 0.018319491939423546 10.97624455801628 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2167 5.127295097482492 No Hit CCCATGTACTCTGCGTTGATACCAC 1437 3.4000567859170925 No Hit GAGTACATGGGAAGCAGTGGTATCA 1035 2.4488926746166952 No Hit GTATCAACGCAGAGTACTTTTTTTT 934 2.2099186068521672 No Hit GGTATCAACGCAGAGTACTTTTTTT 737 1.7438008707173953 No Hit TATCAACGCAGAGTACTTTTTTTTT 729 1.7248722316865417 No Hit CATGTACTCTGCGTTGATACCACTG 619 1.4646034450123036 No Hit ACGCAGAGTACATGGGAAGCAGTGG 600 1.4196479273140261 No Hit TATCAACGCAGAGTACATGGGAAGC 596 1.4101836077985992 No Hit GGTATCAACGCAGAGTACATGGGAA 540 1.2776831345826234 No Hit GTATCAACGCAGAGTACATGGGAAG 519 1.2279954571266325 No Hit GCGTTGATACCACTGCTTCCCATGT 500 1.183039939428355 No Hit CTTATACACATCTCCGAGCCCACGA 422 0.9984857088775316 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 400 0.9464319515426841 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 394 0.9322354722695438 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 386 0.9133068332386902 No Hit GTGGTATCAACGCAGAGTACATGGG 375 0.8872799545712663 No Hit ACTCTGCGTTGATACCACTGCTTCC 374 0.8849138746924097 No Hit ATACACATCTCCGAGCCCACGAGAC 291 0.6885292447473027 No Hit GGGAAGCAGTGGTATCAACGCAGAG 271 0.6412076471701684 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 254 0.6009842892296045 No Hit GCAGTGGTATCAACGCAGAGTACAT 252 0.596252129471891 No Hit CAGTGGTATCAACGCAGAGTACATG 247 0.5844217300776073 No Hit CATGGGAAGCAGTGGTATCAACGCA 238 0.5631270111678971 No Hit GTACTCTGCGTTGATACCACTGCTT 218 0.5158054135907628 No Hit GCTTCCCATGTACTCTGCGTTGATA 216 0.5110732538330494 No Hit GCAGAGTACATGGGAAGCAGTGGTA 215 0.5087071739541927 No Hit ACATGGGAAGCAGTGGTATCAACGC 207 0.48977853492333906 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 200 0.47321597577134206 No Hit ATACCACTGCTTCCCATGTACTCTG 199 0.47084989589248527 No Hit GATACCACTGCTTCCCATGTACTCT 198 0.46848381601362865 No Hit ACACATCTCCGAGCCCACGAGACCT 193 0.4566534166193451 No Hit AAGCAGTGGTATCAACGCAGAGTAC 185 0.4377247775884914 No Hit ATGGGAAGCAGTGGTATCAACGCAG 183 0.43299261783077797 No Hit TACCACTGCTTCCCATGTACTCTGC 157 0.37147454098050353 No Hit CTGCTTCCCATGTACTCTGCGTTGA 154 0.3643763013439334 No Hit CCACTGCTTCCCATGTACTCTGCGT 142 0.33598334279765285 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 135 0.31942078364565585 No Hit GTGGTATCAACGCAGAGTACTTTTT 126 0.2981260647359455 No Hit GTTGATACCACTGCTTCCCATGTAC 125 0.29575998485708876 No Hit TGATACCACTGCTTCCCATGTACTC 124 0.29339390497823203 No Hit ATCAACGCAGAGTACATGGGAAGCA 114 0.26973310618966495 No Hit CCATGTACTCTGCGTTGATACCACT 105 0.2484383872799546 No Hit ATCAACGCAGAGTACTTTTTTTTTT 102 0.24134014764338446 No Hit CTTCCCATGTACTCTGCGTTGATAC 102 0.24134014764338446 No Hit GGAAGCAGTGGTATCAACGCAGAGT 101 0.2389740677645277 No Hit TTCCCATGTACTCTGCGTTGATACC 99 0.23424190800681433 No Hit GGTATCAACGCAGAGTACATGGGGA 79 0.1869203104296801 No Hit CATCTCCGAGCCCACGAGACCTCTC 76 0.17982207079310997 No Hit GTACATGGGGAGGAAAAAAAAAAAA 75 0.17745599091425326 No Hit GGTATCAACGCAGAGTACATGGGGG 75 0.17745599091425326 No Hit GTACATGGTAAGCAGTGGTATCAAC 71 0.16799167139882643 No Hit TGGGAAGCAGTGGTATCAACGCAGA 65 0.15379519212568615 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 64 0.15142911224682945 No Hit GTACATGGGTAAGCAGTGGTATCAA 62 0.14669695248911602 No Hit AAAAAGTACTCTGCGTTGATACCAC 61 0.14433087261025931 No Hit ACCATGTACTCTGCGTTGATACCAC 57 0.13486655309483248 No Hit GGTAAGCAGTGGTATCAACGCAGAG 57 0.13486655309483248 No Hit GTACATGGGGAAAAAAAAAAAAAAA 55 0.13013439333711907 No Hit AGTGGTATCAACGCAGAGTACATGG 55 0.13013439333711907 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 53 0.12540223357940564 No Hit AACGCAGAGTACTTTTTTTTTTTTT 51 0.12067007382169223 No Hit CACATCTCCGAGCCCACGAGACCTC 51 0.12067007382169223 No Hit TCTCCGAGCCCACGAGACCTCTCTA 50 0.11830399394283551 No Hit TCAACGCAGAGTACATGGGAAGCAG 49 0.11593791406397881 No Hit AAAGTACTCTGCGTTGATACCACTG 48 0.1135718341851221 No Hit AACGCAGAGTACATGGGAAGCAGTG 45 0.10647359454855196 No Hit TCAACGCAGAGTACTTTTTTTTTTT 43 0.10174143479083854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 45 1.5915266E-6 19.0 1 TTATACA 60 7.176095E-8 17.416666 2 TATACAC 55 5.5819146E-7 17.272728 3 GAGACCT 55 1.8901342E-4 13.818182 19 ATACACA 75 1.39977765E-5 12.666667 4 CGAGACC 60 3.947657E-4 12.666666 18 TACACAT 85 5.0446117E-5 11.17647 5 GAAGCAG 515 0.0 9.223301 9 GGAAGCA 535 0.0 9.056075 8 CAGTGGT 590 0.0 8.372882 13 AAGCAGT 590 0.0 8.372882 10 CACATCT 115 0.0010557375 8.260869 7 ACATCTC 115 0.0010557375 8.260869 8 ACACATC 115 0.0010557375 8.260869 6 AGCAGTG 600 0.0 8.233334 11 GGGAAGC 600 0.0 8.233334 7 AGTGGTA 605 0.0 8.165289 14 ATCTCCG 120 0.0016082015 7.916666 10 CATCTCC 120 0.0016082015 7.916666 9 GCAGTGG 645 0.0 7.6589146 12 >>END_MODULE