FastQCFastQC Report
Thu 26 May 2016
SRR1512548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033870
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55990.2752879977579688No Hit
CTGTAGGACGTGGAATATGGCAAGA50440.24800011800164218No Hit
GTCCTAAAGTGTGTATTTCTCATTT50390.24775428124708068No Hit
CTTTAGGACGTGAAATATGGCGAGG42840.21063293130829402No Hit
GTATCAACGCAGAGTACTTTTTTTT42060.20679787793713464No Hit
GTCCTACAGTGTGCATTTCTCATTT30350.1492229100188311No Hit
GGTATCAACGCAGAGTACTTTTTTT25450.125130908071804No Hit
TATCAACGCAGAGTACTTTTTTTTT24350.11972249947145097No Hit
CTGAAGGACCTGGAATATGGCGAGA22880.11249489888734285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10150.013.162494
CGGTAGG852.128365E-612.9903561
GGCGAGG12750.011.57993119
CCACCTT6550.011.42999313
ATTTAGA7000.011.0418021
GTCCTAA14400.011.0139191
CACCTTT7150.011.003448514
ACCGTGA700.001555151310.8030858
TTTAGAA7800.010.6898862
AGGACCG803.8642084E-410.6595165
AGGACCT20200.010.6477895
AATGTCC7750.010.6114188
ACCTTTT7100.010.54691715
GGTATCA12000.010.4562521
TGTAGGA32100.010.4492042
GTCCTAC29300.010.4491151
GGACCTG19350.010.332056
TGGCGAG30900.010.31968718
GTCCTAT2350.010.2515771
GTAGGAC32350.010.2512963
Produced by FastQC (version 0.11.5)