FastQCFastQC Report
Thu 26 May 2016
SRR1512548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033870
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58890.2895465295225359No Hit
CTGTAGGACGTGGAATATGGCAAGA56180.27622217742530253No Hit
GTATCAACGCAGAGTACTTTTTTTT55640.2735671404760383No Hit
GTCCTAAAGTGTGTATTTCTCATTT51970.2555227226912241No Hit
CTTTAGGACGTGAAATATGGCGAGG48220.23708496609911156No Hit
GGTATCAACGCAGAGTACTTTTTTT36840.18113252076091393No Hit
TATCAACGCAGAGTACTTTTTTTTT36570.17980500228628182No Hit
GTCCTACAGTGTGCATTTCTCATTT29830.14666620777139147No Hit
CTGAAGGACCTGGAATATGGCGAGA28070.13801275401082666No Hit
CTGTAGGACCTGGAATATGGCGAGA24860.12223003436797829No Hit
TTTCTAAATTTTCCACCTTTTTCAG22080.10856151081435883No Hit
ATTTAGAAATGTCCACTGTAGGACG21750.10693898823425294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTT250.0060460218.99274819
TAGGACC8300.013.5091724
CCGGTTT1550.013.48270212
TCCAACG2750.013.467916518
GCCGGTT1500.013.29917311
CCAACGT1001.0131771E-813.29492419
CCAACGA1800.013.18940819
CGGTTTC1700.012.29214513
TGCCGGT1603.6379788E-1211.87455510
TGGCGAA1302.6320777E-911.68813218
ACCTTTT7650.011.54517915
GGCGAGG13750.011.53377819
CCTACCG752.0699306E-411.4026573
AGGCCGC752.07277E-411.4009736
AATGTCC7700.011.103488
AGGACCT18500.010.8892925
CCACCTT7900.010.82104813
TGCGTTG2650.010.75378412
CACCTTT8350.010.69105414
AGGACGT32000.010.6902545
Produced by FastQC (version 0.11.5)