FastQCFastQC Report
Thu 26 May 2016
SRR1512547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629140
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37180.22821856930650528No Hit
GTCCTACAGTGGACATTTCTAAATT27380.16806413199602244No Hit
CTGTAGGACGTGGAATATGGCAAGA25860.15873405600500878No Hit
GTCCTAAAGTGTGTATTTCTCATTT24940.15308690474728998No Hit
CTTTAGGACGTGAAATATGGCGAGG22510.13817105957744577No Hit
GGTATCAACGCAGAGTACTTTTTTT22390.13743447463078678No Hit
TATCAACGCAGAGTACTTTTTTTTT21210.13019138932197355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG550.00313256312.0576144
TAGGACC4450.011.9221354
GGGTAAC1051.7898874E-611.4713041
GTATAGG1757.2759576E-1211.4713031
GCTATAC1105.1462666E-711.1967023
AGGACCG951.402387E-510.969715
GGTCTAT1103.129664E-610.9498811
AGGACGT14850.010.7816775
GGCGAGG7850.010.61086419
TGTAGGA16150.010.5594042
GTATAAA3050.010.5310321
GGACGTG14850.010.4630156
GTGTTAG1551.729677E-810.3611771
GACGTGG8350.010.3251037
GTATTAA2250.010.2604441
TGGCGAG15400.010.20136218
GTAGGAC15650.010.1703153
TCTAGAC1502.5493136E-810.10574153
ACCGTGC750.002755713410.0845868
TTAGGAC9400.010.0788643
Produced by FastQC (version 0.11.5)