FastQCFastQC Report
Thu 26 May 2016
SRR1512547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629140
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45070.2766490295493328No Hit
TATCAACGCAGAGTACTTTTTTTTT29410.18052469401033674No Hit
GGTATCAACGCAGAGTACTTTTTTT29350.18015640153700724No Hit
GTCCTACAGTGGACATTTCTAAATT28160.17285193414930577No Hit
CTGTAGGACGTGGAATATGGCAAGA27030.16591575923493376No Hit
GTCCTAAAGTGTGTATTTCTCATTT25780.1582429993739028No Hit
CTTTAGGACGTGAAATATGGCGAGG25170.15449869256171966No Hit
GTACATGGGAAGCAGTGGTATCAAC16600.10189425095449134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5650.013.45395854
TTTAGAC905.394704E-712.6687553
CTAGACA1604.5474735E-1111.2834574
AGGACCT11100.011.2139045
GGCGAGG6650.010.99573619
GGACCTG10400.010.6876216
TGCGCCA909.5196316E-510.55502712
TGTAGGA16400.010.544422
CTGTAGG16750.010.4490931
GTAGGAC16800.010.4064773
GGTATCA13150.010.3439371
AAATGTC4050.010.3214217
TCTAGAC1752.3464963E-1010.3159863
TGGCGAG15750.010.3109218
TAGAAAT4700.010.3105274
TCGGAGC750.002644068810.1350043
GTGCGCC951.6437448E-410.000113511
AATGTCC4500.09.9223348
GGCGAGA9750.09.83721119
TGACGAA1554.06053E-89.80656210
Produced by FastQC (version 0.11.5)