FastQCFastQC Report
Thu 26 May 2016
SRR1512546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590789
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46920.2949479786445594No Hit
GTCCTAAAGTGTGTATTTCTCATTT43110.2709975993044961No Hit
CTGTAGGACGTGGAATATGGCAAGA39760.24993886681388922No Hit
CTTTAGGACGTGAAATATGGCGAGG37930.23843514130409502No Hit
GTATCAACGCAGAGTACTTTTTTTT37020.23271470949321377No Hit
GTCCTACAGTGTGCATTTCTCATTT27820.17488177250408446No Hit
GGTATCAACGCAGAGTACTTTTTTT22730.14288507149596835No Hit
TATCAACGCAGAGTACTTTTTTTTT22020.13842187744572032No Hit
GTACATGGGAAGCAGTGGTATCAAC20440.1284896991367177No Hit
CTGAAGGACCTGGAATATGGCGAGA19850.12478084774284963No Hit
CTGTAGGACCTGGAATATGGCGAGA19750.12415222886253299No Hit
CCCATGTACTCTGCGTTGATACCAC17660.11101409426391559No Hit
GTCCTTCAGTGTGCATTTCTCATTT17190.10805958552642744No Hit
ATTTAGAAATGTCCACTGTAGGACG16230.10202484427538788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTG400.005376787414.2106915
ACCGTGC400.0054446714.1838388
GTCTAAG1700.014.1418331
TAGGACC7700.014.0261384
GCGTGCG552.032035E-413.7596599
AAATGTC5900.012.5254687
GTCCTAT2000.012.5214161
CACCTTT6150.012.33141714
AATGTCC6100.012.2461568
TCGAACT1103.9910447E-812.05147319
CTCGAAC1103.9963197E-812.05033218
TAGAAAT6350.011.6371024
CCACCTT6350.011.34157813
GGCGAGG11550.011.23164519
GTCCAGG2950.011.20560551
GAAATGT6350.011.1895216
GTGTAGG2600.011.1729551
CTAGGAC3400.011.1459933
GTCCTAC22950.011.0428511
GGACCTG15350.010.924186
Produced by FastQC (version 0.11.5)